Infection and Immunity, October 2004, p. 6157-6159, Vol. 72, No. 10
0019-9567/04/$08.00+0 DOI: 10.1128/IAI.72.10.6157-6159.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
A Calcium-Calmodulin Antagonist Blocks Experimental Vibrio vulnificus Cytolysin-Induced Lethality in an Experimental Mouse Model
Young-Rae Lee,1 Kwang-Hyun Park,1 Zhao-Zhen Lin,1 Young-Jong Kho,1 Jin-Woo Park,1 Hye-Won Rho,1 Bon-Sun Koo,1 Hyung-Rho Kim,1 Eun-Kyung Song,2 Hong-Nu Yu,2 Myung-Kwan Han,2 Seung-Ok Lee,3 Eun-Chung Jhee,4 and Jong-Suk Kim1*
Departments of Biochemistry,1
Microbiology and Immunology,2
Internal Medicine, Chonbuk National University Medical School,3
Department of Biochemistry, Chonbuk National University Dental School, Chonju, Republic of Korea4
Received 22 April 2004/
Returned for modification 9 June 2004/
Accepted 28 June 2004
We demonstrated that trifluoperazine, a calcium-calmodulin antagonist, blocked the hyperpermeability induced by Vibrio vulnificus cytolysin in in vitro-modeled endothelium and prevented the deaths of mice. Furthermore, compared to tetracycline alone, tetracycline combined with trifluoperazine enhanced the survival rate of V. vulnificus-infected mice, indicating the role of the cytolysin as an important factor in pathogenesis.
* Corresponding author. Mailing address: Department of Biochemistry, Chonbuk National University Medical School, Chonju 560-182, Republic of Korea. Phone: 82-63-270-3085. Fax: 82-63-274-9833. E-mail: jsukim{at}moak.chonbuk.ac.kr.
Editor: J. T. Barbieri
Infection and Immunity, October 2004, p. 6157-6159, Vol. 72, No. 10
0019-9567/04/$08.00+0 DOI: 10.1128/IAI.72.10.6157-6159.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Copyright © 2004 by the American Society for Microbiology. All rights reserved.