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Infection and Immunity, November 2004, p. 6480-6491, Vol. 72, No. 11
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.11.6480-6491.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Combined Vaccine Regimen Based on Parenteral Priming with a DNA Vaccine and Administration of an Oral Booster Consisting of a Recombinant Salmonella enterica Serovar Typhimurium Vaccine Strain for Immunization against Infection with Human-Derived Enterotoxigenic Escherichia coli Strains

Marcio O. Lásaro,1,{dagger} Wilson B. Luiz,1 Maria E. Sbrogio-Almeida,2 Lucilia S. Nishimura,3 Beatriz E. C. Guth,3 and Luis C. S. Ferreira1*

Department of Microbiology, Biomedical Sciences Institute, University of São Paulo,1 Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo,3 Division of Technological Development and Production, Butantan Institute, São Paulo, Brazil2

Received 21 January 2004/ Returned for modification 12 March 2004/ Accepted 6 May 2004

Repeated evidence has demonstrated that combined primer-booster immunization regimens can improve both secreted and humoral immune responses to antigens derived from viral, bacterial, and parasitic pathogens. For the present work, we evaluated the synergic serum immunoglobulin G (IgG) and fecal IgA antibody responses elicited in BALB/c mice who were intramuscularly primed with a DNA vaccine, pRECFA, followed by oral boosting with an attenuated Salmonella enterica serovar Typhimurium vaccine (HG3) strain, with both vaccines encoding the structural subunit (CfaB) of the CFA/I fimbriae produced by human-derived enterotoxigenic Escherichia coli (ETEC) strains. The immunological properties of the vaccine regimen were evaluated according to the order of the administered vaccines, the nature of the oral antigen carrier, the age of the vaccinated animals, the interval between the priming and boosting doses, and the amount of injected DNA. The production of gamma interferon and the IgG2a subclass in serum indicated that mice immunized with the primer-booster regimen developed prevailing type 1 T-cell-dependent immune responses. The synergic effect of the vaccine regimen on the induced antibody responses was also revealed by its ability to block the adhesive properties of CFA/I fimbriae expressed by live bacteria, as shown by the inhibition of Caco-2 cell and human erythrocyte binding. Moreover, DBA2 newborn mice were protected from lethal challenges with a CFA/I+ ETEC strain after the incubation of live bacteria with serum samples harvested from mice who were subjected to the primer-booster regimen. We propose, therefore, that the DNA primer-Salmonella booster regimen represents an alternative for the development of vaccines requiring both mucosal and systemic antibody responses for immunological protection.

* Corresponding author. Mailing address: Av. Prof. Lineu Prestes 1374, Departamento de Microbiologia, São Paulo Cidade Universitária, 05508-000 São Paulo, Brazil. Phone: 5511-3091-7356. Fax: 5511-3091-7354. E-mail: lcsf{at}usp.br.

Editor: J. T. Barbieri

{dagger} Present address: The Wistar Institute, Philadelphia, Penn.

Infection and Immunity, November 2004, p. 6480-6491, Vol. 72, No. 11
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.11.6480-6491.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bentancor, L. V., Bilen, M., Brando, R. J. F., Ramos, M. V., Ferreira, L. C. S., Ghiringhelli, P. D., Palermo, M. S. (2009). A DNA Vaccine Encoding the Enterohemorragic Escherichia coli Shiga-Like Toxin 2 A2 and B Subunits Confers Protective Immunity to Shiga Toxin Challenge in the Murine Model. CVI 16: 712-718 [Abstract] [Full Text]  


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