This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, K.-S.
Right arrow Articles by Honda, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, K.-S.
Right arrow Articles by Honda, T.

 Previous Article  |  Next Article 

Infection and Immunity, November 2004, p. 6659-6665, Vol. 72, No. 11
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.11.6659-6665.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Functional Characterization of Two Type III Secretion Systems of Vibrio parahaemolyticus

Kwon-Sam Park,1 Takahiro Ono,1 Mitsuhiro Rokuda,1 Myoung-Ho Jang,2 Kazuhisa Okada,1 Tetsuya Iida,1* and Takeshi Honda1

Department of Bacterial Infections,1 Department of Mucosal Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan2

Received 9 May 2004/ Returned for modification 9 June 2004/ Accepted 10 August 2004

Vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. Recent genome sequencing of the clinical V. parahaemolyticus strain RIMD2210633 identified two sets of genes for the type III secretion system (TTSS), TTSS1 and TTSS2. Here, we constructed a series of mutant strains from RIMD2210633 to determine whether the two putative TTSS apparatus are functional. The cytotoxic activity of mutant strains having a deletion in one of the TTSS1 genes was significantly decreased compared with that of the parent and TTSS2-related mutant strains. In an enterotoxicity assay with the rabbit ileal loop test, intestinal fluid accumulation was diminished by deletion of the TTSS2-related genes while TTSS1-related mutants caused a level of fluid accumulation similar to that of the parent. VopD, a protein encoded in the proximity of the TTSS1 region and a homologue of the Yersinia YopD, was secreted in a TTSS1-dependent manner. In contrast, VopP, which is encoded by a pathogenicity island on chromosome 2 and is homologous to the Yersinia YopP, was secreted via the TTSS2 pathway. These results provide evidence that V. parahaemolyticus TTSSs function as secretion systems and may have a role in the pathogenicity of the organism. This is the first report of functional TTSSs in Vibrio species. The presence of TTSS apparatus gene homologues was demonstrated in other vibrios, such as Vibrio alginolyticus, Vibrio harveyi, and Vibrio tubiashii, suggesting that some other vibrios also contain TTSS and that the TTSS has a role in protein secretion in those organisms during interaction with eukaryotic cells.

* Corresponding author. Mailing address: Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Phone: 81 6 6879 8278. Fax: 81 6 6879 8277. E-mail: iida{at}biken.osaka-u.ac.jp.

Editor: D. L. Burns

Infection and Immunity, November 2004, p. 6659-6665, Vol. 72, No. 11
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.11.6659-6665.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hazen, T. H., Martinez, R. J., Chen, Y., Lafon, P. C., Garrett, N. M., Parsons, M. B., Bopp, C. A., Sullards, M. C., Sobecky, P. A. (2009). Rapid Identification of Vibrio parahaemolyticus by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Appl. Environ. Microbiol. 75: 6745-6756 [Abstract] [Full Text]  
  • Hasegawa, H., Hase, C. C. (2009). The extracellular metalloprotease of Vibrio tubiashii directly inhibits its extracellular haemolysin. Microbiology 155: 2296-2305 [Abstract] [Full Text]  
  • Zhou, X., Konkel, M. E., Call, D. R. (2009). Type III secretion system 1 of Vibrio parahaemolyticus induces oncosis in both epithelial and monocytic cell lines. Microbiology 155: 837-851 [Abstract] [Full Text]  
  • Okada, N., Iida, T., Park, K.-S., Goto, N., Yasunaga, T., Hiyoshi, H., Matsuda, S., Kodama, T., Honda, T. (2009). Identification and Characterization of a Novel Type III Secretion System in trh-Positive Vibrio parahaemolyticus Strain TH3996 Reveal Genetic Lineage and Diversity of Pathogenic Machinery beyond the Species Level. Infect. Immun. 77: 904-913 [Abstract] [Full Text]  
  • Yarbrough, M. L., Li, Y., Kinch, L. N., Grishin, N. V., Ball, H. L., Orth, K. (2009). AMPylation of Rho GTPases by Vibrio VopS Disrupts Effector Binding and Downstream Signaling. Science 323: 269-272 [Abstract] [Full Text]  
  • Laskowski-Arce, M. A., Orth, K. (2008). Acanthamoeba castellanii Promotes the Survival of Vibrio parahaemolyticus. Appl. Environ. Microbiol. 74: 7183-7188 [Abstract] [Full Text]  
  • Kodama, T., Hiyoshi, H., Gotoh, K., Akeda, Y., Matsuda, S., Park, K.-S., Cantarelli, V. V., Iida, T., Honda, T. (2008). Identification of Two Translocon Proteins of Vibrio parahaemolyticus Type III Secretion System 2. Infect. Immun. 76: 4282-4289 [Abstract] [Full Text]  
  • Burdette, D. L., Yarbrough, M. L., Orvedahl, A., Gilpin, C. J., Orth, K. (2008). Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis. Proc. Natl. Acad. Sci. USA 105: 12497-12502 [Abstract] [Full Text]  
  • Kam, K. M., Luey, C. K. Y., Parsons, M. B., Cooper, K. L. F., Nair, G. B., Alam, M., Islam, M. A., Cheung, D. T. L., Chu, Y. W., Ramamurthy, T., Pazhani, G. P., Bhattacharya, S. K., Watanabe, H., Terajima, J., Arakawa, E., Ratchtrachenchai, O.-A., Huttayananont, S., Ribot, E. M., Gerner-Smidt, P., Swaminathan, B., for the Vibrio parahaemolyticus PulseNet PFGE Prot, (2008). Evaluation and Validation of a PulseNet Standardized Pulsed-Field Gel Electrophoresis Protocol for Subtyping Vibrio parahaemolyticus: an International Multicenter Collaborative Study. J. Clin. Microbiol. 46: 2766-2773 [Abstract] [Full Text]  
  • Hasegawa, H., Lind, E. J., Boin, M. A., Hase, C. C. (2008). The Extracellular Metalloprotease of Vibrio tubiashii Is a Major Virulence Factor for Pacific Oyster (Crassostrea gigas) Larvae. Appl. Environ. Microbiol. 74: 4101-4110 [Abstract] [Full Text]  
  • Sugiyama, T., Iida, T., Izutsu, K., Park, K.-S., Honda, T. (2008). Precise Region and the Character of the Pathogenicity Island in Clinical Vibrio parahaemolyticus Strains. J. Bacteriol. 190: 1835-1837 [Abstract] [Full Text]  
  • Izutsu, K., Kurokawa, K., Tashiro, K., Kuhara, S., Hayashi, T., Honda, T., Iida, T. (2008). Comparative Genomic Analysis Using Microarray Demonstrates a Strong Correlation between the Presence of the 80-Kilobase Pathogenicity Island and Pathogenicity in Kanagawa Phenomenon-Positive Vibrio parahaemolyticus Strains. Infect. Immun. 76: 1016-1023 [Abstract] [Full Text]  
  • Liverman, A. D. B., Cheng, H.-C., Trosky, J. E., Leung, D. W., Yarbrough, M. L., Burdette, D. L., Rosen, M. K., Orth, K. (2007). Arp2/3-independent assembly of actin by Vibrio type III effector VopL. Proc. Natl. Acad. Sci. USA 104: 17117-17122 [Abstract] [Full Text]  
  • Coburn, B., Sekirov, I., Finlay, B. B. (2007). Type III Secretion Systems and Disease. Clin. Microbiol. Rev. 20: 535-549 [Abstract] [Full Text]  
  • Coutard, F., Lozach, S., Pommepuy, M., Hervio-Heath, D. (2007). Real-Time Reverse Transcription-PCR for Transcriptional Expression Analysis of Virulence and Housekeeping Genes in Viable but Nonculturable Vibrio parahaemolyticus after Recovery of Culturability. Appl. Environ. Microbiol. 73: 5183-5189 [Abstract] [Full Text]  
  • Meador, C. E., Parsons, M. M., Bopp, C. A., Gerner-Smidt, P., Painter, J. A., Vora, G. J. (2007). Virulence Gene- and Pandemic Group-Specific Marker Profiling of Clinical Vibrio parahaemolyticus Isolates. J. Clin. Microbiol. 45: 1133-1139 [Abstract] [Full Text]  
  • Nair, G. B., Ramamurthy, T., Bhattacharya, S. K., Dutta, B., Takeda, Y., Sack, D. A. (2007). Global Dissemination of Vibrio parahaemolyticus Serotype O3:K6 and Its Serovariants. Clin. Microbiol. Rev. 20: 39-48 [Abstract] [Full Text]  
  • Bhattacharjee, R. N., Park, K.-S., Kumagai, Y., Okada, K., Yamamoto, M., Uematsu, S., Matsui, K., Kumar, H., Kawai, T., Iida, T., Honda, T., Takeuchi, O., Akira, S. (2006). VP1686, a Vibrio Type III Secretion Protein, Induces Toll-like Receptor-independent Apoptosis in Macrophage through NF-{kappa}B Inhibition. J. Biol. Chem. 281: 36897-36904 [Abstract] [Full Text]  
  • Thompson, F. L., Klose, K. E., the AVIB Group, (2006). Vibrio2005: the First International Conference on the Biology of Vibrios. J. Bacteriol. 188: 4592-4596 [Full Text]  
  • Wang, H.-z., Wong, M. M. L., O'Toole, D., Mak, M. M. H., Wu, R. S. S., Kong, R. Y. C. (2006). Identification of a DNA Methyltransferase Gene Carried on a Pathogenicity Island-Like Element (VPAI) in Vibrio parahaemolyticus and Its Prevalence among Clinical and Environmental Isolates.. Appl. Environ. Microbiol. 72: 4455-4460 [Abstract] [Full Text]  
  • Ono, T., Park, K.-S., Ueta, M., Iida, T., Honda, T. (2006). Identification of Proteins Secreted via Vibrio parahaemolyticus Type III Secretion System 1. Infect. Immun. 74: 1032-1042 [Abstract] [Full Text]  
  • Troisfontaines, P., Cornelis, G. R. (2005). Type III Secretion: More Systems Than You Think. Physiology 20: 326-339 [Abstract] [Full Text]  
  • Dziejman, M., Serruto, D., Tam, V. C., Sturtevant, D., Diraphat, P., Faruque, S. M., Rahman, M. H., Heidelberg, J. F., Decker, J., Li, L., Montgomery, K. T., Grills, G., Kucherlapati, R., Mekalanos, J. J. (2005). Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system. Proc. Natl. Acad. Sci. USA 102: 3465-3470 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»