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Infection and Immunity, December 2004, p. 7164-7171, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.7164-7171.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Human Antibody Responses to a Chlamydia-Secreted Protease Factor

Jyotika Sharma,1 Anthony M. Bosnic,1 Jeanna M. Piper,2 and Guangming Zhong1*

Departments of Microbiology and Immunology,1 Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, Texas2

Received 12 May 2004/ Returned for modification 22 June 2004/ Accepted 11 August 2004

We have previously identified a chlamydia-secreted protein (designated chlamydial proteasome/protease-like activity factor, or CPAF) in the cytosol of chlamydia-infected cells. Although CPAF is known to degrade host transcription factors required for major histocompatibility complex antigen expression in cultured cells, it is not clear whether CPAF is produced and maintains similar functions in humans infected with chlamydial organisms. We now report that CPAF does not preexist in chlamydial organisms and that CPAF synthesis requires live organism replication in cultured cells. Mice inoculated with live, but not mice inoculated with dead, chlamydial organisms produced a strong antibody response to CPAF, correlating CPAF-specific antibody production with CPAF synthesis in animals. Sera from women diagnosed with Chlamydia trachomatis cervicitis displayed higher levels of antibodies to CPAF than to either chlamydial major outer membrane protein or heat shock protein 60, suggesting that CPAF is both produced and immunogenic during human chlamydial infection.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229. Phone: (210) 567-1169. Fax: (210) 567-0293. E-mail: Zhongg{at}UTHSCSA.EDU.

Editor: J. T. Barbieri

Infection and Immunity, December 2004, p. 7164-7171, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.7164-7171.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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