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Infection and Immunity, December 2004, p. 7231-7239, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.7231-7239.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Differential Regulation of Inflammatory Cytokine Secretion by Human Dendritic Cells upon Chlamydia trachomatis Infection

Ana Gervassi,1,2 Mark R. Alderson,1 Robert Suchland,3 Jean François Maisonneuve,1 Kenneth H. Grabstein,1 and Peter Probst1*

Corixa Corporation,1 Department of Pathobiology,2 Division of Allergy and Infectious Diseases, School of Medicine, University of Washington, Seattle, Washington3

Received 14 July 2003/ Returned for modification 30 September 2003/ Accepted 30 August 2004

Chlamydia trachomatis is an obligate intracellular gram-negative bacterium responsible for a wide spectrum of diseases in humans. Both genital and ocular C. trachomatis infections are associated with tissue inflammation and pathology. Dendritic cells (DC) play an important role in both innate and adaptive immune responses to microbial pathogens and are a source of inflammatory cytokines. To determine the potential contribution of DC to the inflammatory process, human DC were infected with C. trachomatis serovar E or L2. Both C. trachomatis serovars were found to infect and replicate in DC. Upon infection, DC up-regulated the expression of costimulatory (B7-1) and cell adhesion (ICAM-1) molecules. Furthermore, chlamydial infection induced the secretion of interleukin-1ß (IL-1ß), IL-6, IL-8, IL-12p70, IL-18, and tumor necrosis factor alpha (TNF-{alpha}). The mechanisms involved in Chlamydia-induced IL-1ß and IL-18 secretion differed from those of the other cytokines. Chlamydia-induced IL-1ß and IL-18 secretion required infection with viable bacteria and was associated with the Chlamydia-induced activation of caspase-1 in infected host cells. In contrast, TNF-{alpha} and IL-6 secretion did not require that the Chlamydia be viable, suggesting that there are at least two mechanisms involved in the Chlamydia-induced cytokine secretion in DC. Interestingly, an antibody to Toll-like receptor 4 inhibited Chlamydia-induced IL-1ß, IL-6, and TNF-{alpha} secretion. The data herein demonstrate that DC can be infected by human C. trachomatis serovars and that chlamydial components regulate the secretion of various cytokines in DC. Collectively, these data suggest that DC play a role in the inflammatory processes caused by chlamydial infections.

* Corresponding author. Mailing address: Corixa Corporation, 1900 9th Ave., Suite 1100, Seattle, WA 98101. Phone: (206) 366-3760. Fax: (206) 366-4760. E-mail: peter.probst{at}corixa.com.

Editor: S. H. E. Kaufmann

Infection and Immunity, December 2004, p. 7231-7239, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.7231-7239.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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