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Infection and Immunity, December 2004, p. 7342-7345, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.7342-7345.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Intranasal Vaccination with Streptococcal Fibronectin Binding Protein Sfb1 Fails To Prevent Growth and Dissemination of Streptococcus pyogenes in a Murine Skin Infection Model

J. McArthur,^1,2 E. Medina,³ A. Mueller,³ J. Chin,⁴ B. J. Currie,^5,6 K. S. Sriprakash,^1,5,6 S. R. Talay,³ G. S. Chhatwal,³ and M. J. Walker^2*

Bacterial Pathogenesis Laboratory, Queensland Institute of Medical Research, Brisbane,¹ NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden,⁴ Menzies School of Health Research, Charles Darwin University,⁵ Co-operative Research Centre for Aboriginal and Tropical Health, Darwin,⁶ Department of Biological Sciences, University of Wollongong, Wollongong, Australia,² GBF National Research Center for Biotechnology, Braunschweig, Germany³

Received 27 April 2004/ Returned for modification 24 June 2004/ Accepted 30 August 2004

Fibronectin binding protein F1 (Sfb1) of Streptococcus pyogenes (group A streptococcus [GAS]) is a well-characterized adhesin that has been shown to induce protection in mice against a lethal intranasal GAS challenge after intranasal immunization with cholera toxin B subunit (CTB) as adjuvant. With a murine skin infection model, we have shown that Sfb1/CTB vaccination neither elicits opsonizing antibodies nor prevents systemic bacterial growth and dissemination to internal organs after a subcutaneous GAS challenge. These results indicate that an Sfb1-based vaccine should be complemented with additional protective antigens in order to be used in areas such as the tropical north of Australia, where the skin is the primary route of entry for invasive streptococcal diseases.

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* Corresponding author. Mailing address: Department of Biological Sciences, University of Wollongong, Wollongong, N.S.W. 2522, Australia. Phone: 0061-242-213439. Fax: 0061-242-214135. E-mail: mwalker{at}uow.edu.au.

Editor: S. H. E. Kaufmann

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Infection and Immunity, December 2004, p. 7342-7345, Vol. 72, No. 12
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.12.7342-7345.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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