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Infection and Immunity, February 2004, p. 1174-1180, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.1174-1180.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

An msbB Homologue Carried in Plasmid pO157 Encodes an Acyltransferase Involved in Lipid A Biosynthesis in Escherichia coli O157:H7

Sang-Hyun Kim,1 Wenyi Jia,2 Russell E. Bishop,2 and Carlton Gyles1*

Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1,1 Departments of Laboratory Medicine and Pathobiology and of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A82

Received 22 April 2003/ Returned for modification 24 July 2003/ Accepted 22 October 2003

Escherichia coli O157:H7 carries a chromosomal msbB1 and a plasmid-encoded msbB2 gene. We characterized msbB2 function as a homologue of msbB1 by examination of wild-type organisms and mutant strains that lacked functional msbB1, msbB2, and both msbB1 and msbB2. The msbB double-mutant strain generated pentaacyl lipid A, while the single-mutant strains synthesized hexaacyl lipid A. Complementation with overexpressed msbB2 converted pentaacyl into hexaacyl lipid A in the double-mutant strain. The transcription of both msbB genes occurred simultaneously. Lack of MsbB2 activity slightly increased the microheterogeneity of the lipid A species. These results suggest that the msbB2 gene plays a role not only in the routine generation of fully hexaacylated lipid A but also in suppressing the microheterogeneity of lipid A species, the endotoxic determinant of the organism.

* Corresponding author. Mailing address: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1. Phone: (519) 824-4120, ext. 54715. Fax: (519) 767-0809. E-mail: cgyles{at}uoguelph.ca.

Editor: V. J. DiRita

Infection and Immunity, February 2004, p. 1174-1180, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.1174-1180.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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