This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hajishengallis, G. Articles by Genco, R. J. Search for Related Content PubMed PubMed Citation Articles by Hajishengallis, G. Articles by Genco, R. J.

 Previous Article  |  Next Article 

Infection and Immunity, February 2004, p. 1188-1191, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.1188-1191.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Downregulation of the DNA-Binding Activity of Nuclear Factor-B p65 Subunit in Porphyromonas gingivalis Fimbria-Induced Tolerance

Department of Microbiology, Immunology, and Parasitology, Center of Excellence in Oral and Craniofacial Biology, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70119,¹ Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214²

Received 22 September 2003/ Returned for modification 28 October 2003/ Accepted 11 November 2003

Porphyromonas gingivalis fimbriae induce high levels of nuclear factor-B (NF-B)-dependent cytokine release upon primary but not secondary stimulation of monocytic cells (FimA tolerance). In this study, fimbriae induced Toll-like receptor-mediated activation of both p50 and p65 subunits of NF-B upon primary cellular activation. However, activation of the transactivating p65 subunit (but not of the transcriptionally inactive p50 subunit) was significantly inhibited in fimbria-restimulated cells. Moreover, expression of a NF-B-dependent reporter gene was inhibited upon secondary stimulation with fimbriae. NF-B p65 downregulation may thus contribute to induction of FimA tolerance.

Editor: J. T. Barbieri

This article has been cited by other articles:

• Hajishengallis, G., Ratti, P., Harokopakis, E. (2005). Peptide Mapping of Bacterial Fimbrial Epitopes Interacting with Pattern Recognition Receptors. J. Biol. Chem. 280: 38902-38913 [Abstract] [Full Text]  
• Hajishengallis, G., Tapping, R. I., Martin, M. H., Nawar, H., Lyle, E. A., Russell, M. W., Connell, T. D. (2005). Toll-Like Receptor 2 Mediates Cellular Activation by the B Subunits of Type II Heat-Labile Enterotoxins. Infect. Immun. 73: 1343-1349 [Abstract] [Full Text]  
• Graves, D. T., Naguib, G., Huafei Lu, , Desta, T., Amar, S. (2005). Porphyromonas gingivalis fimbriae are pro-inflammatory but do not play a prominent role in the innate immune response to P. gingivalis. Innate Immunity 11: 13-18 [Abstract]  
• Hajishengallis, G., Nawar, H., Tapping, R. I., Russell, M. W., Connell, T. D. (2004). The Type II Heat-Labile Enterotoxins LT-IIa and LT-IIb and Their Respective B Pentamers Differentially Induce and Regulate Cytokine Production in Human Monocytic Cells. Infect. Immun. 72: 6351-6358 [Abstract] [Full Text]