This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hapfelmeier, S.
Right arrow Articles by Hardt, W.-D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hapfelmeier, S.
Right arrow Articles by Hardt, W.-D.

 Previous Article  |  Next Article 

Infection and Immunity, February 2004, p. 795-809, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.795-809.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Role of the Salmonella Pathogenicity Island 1 Effector Proteins SipA, SopB, SopE, and SopE2 in Salmonella enterica Subspecies 1 Serovar Typhimurium Colitis in Streptomycin-Pretreated Mice

Siegfried Hapfelmeier,1 Kristin Ehrbar,1 Bärbel Stecher,1 Manja Barthel,1 Marcus Kremer,2 and Wolf-Dietrich Hardt1*

Institute of Microbiology, ETH Zürich, CH-8092 Zürich, Switzerland,1 Institute of Pathology, Technische Universität München, D-81675 Munich, Germany2

Received 10 July 2003/ Returned for modification 8 September 2003/ Accepted 12 November 2003

Salmonella enterica subspecies 1 serovar Typhimurium (serovar Typhimurium) induces enterocolitis in humans and cattle. The mechanisms of enteric salmonellosis have been studied most extensively in calf infection models. The previous studies established that effector protein translocation into host cells via the Salmonella pathogenicity island 1 (SPI-1) type III secretion system (TTSS) is of central importance in serovar Typhimurium enterocolitis. We recently found that orally streptomycin-pretreated mice provide an alternative model for serovar Typhimurium colitis. In this model the SPI-1 TTSS also plays a key role in the elicitation of intestinal inflammation. However, whether intestinal inflammation in calves and intestinal inflammation in streptomycin-pretreated mice are induced by the same SPI-1 effector proteins is still unclear. Therefore, we analyzed the role of the SPI-1 effector proteins SopB/SigD, SopE, SopE2, and SipA/SspA in elicitation of intestinal inflammation in the murine model. We found that sipA, sopE, and, to a lesser degree, sopE2 contribute to murine colitis, but we could not assign an inflammation phenotype to sopB. These findings are in line with previous studies performed with orally infected calves. Extending these observations, we demonstrated that in addition to SipA, SopE and SopE2 can induce intestinal inflammation independent of each other and in the absence of SopB. In conclusion, our data corroborate the finding that streptomycin-pretreated mice provide a useful model for studying the molecular mechanisms of serovar Typhimurium colitis and are an important starting point for analysis of the molecular events triggered by SopE, SopE2, and SipA in vivo.

* Corresponding author. Mailing address: Institute of Microbiology, ETH Zürich, Schmelzbergstr. 7, 8092 Zürich, Switzerland. Phone: 41-1-632-5143. Fax: 41-1-632-1129. E-mail: hardt{at}micro.biol.ethz.ch.

Editor: B. B. Finlay

Infection and Immunity, February 2004, p. 795-809, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.795-809.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Desin, T. S., Lam, P.-K. S., Koch, B., Mickael, C., Berberov, E., Wisner, A. L. S., Townsend, H. G. G., Potter, A. A., Koster, W. (2009). Salmonella enterica Serovar Enteritidis Pathogenicity Island 1 Is Not Essential for but Facilitates Rapid Systemic Spread in Chickens. Infect. Immun. 77: 2866-2875 [Abstract] [Full Text]  
  • Haneda, T., Winter, S. E., Butler, B. P., Wilson, R. P., Tukel, C., Winter, M. G., Godinez, I., Tsolis, R. M., Baumler, A. J. (2009). The Capsule-Encoding viaB Locus Reduces Intestinal Inflammation by a Salmonella Pathogenicity Island 1-Independent Mechanism. Infect. Immun. 77: 2932-2942 [Abstract] [Full Text]  
  • Winter, S. E., Thiennimitr, P., Nuccio, S.-P., Haneda, T., Winter, M. G., Wilson, R. P., Russell, J. M., Henry, T., Tran, Q. T., Lawhon, S. D., Gomez, G., Bevins, C. L., Russmann, H., Monack, D. M., Adams, L. G., Baumler, A. J. (2009). Contribution of Flagellin Pattern Recognition to Intestinal Inflammation during Salmonella enterica Serotype Typhimurium Infection. Infect. Immun. 77: 1904-1916 [Abstract] [Full Text]  
  • Ashkar, A. A., Reid, S., Verdu, E. F., Zhang, K., Coombes, B. K. (2009). Interleukin-15 and NK1.1+ Cells Provide Innate Protection against Acute Salmonella enterica Serovar Typhimurium Infection in the Gut and in Systemic Tissues. Infect. Immun. 77: 214-222 [Abstract] [Full Text]  
  • Hapfelmeier, S., Muller, A. J., Stecher, B., Kaiser, P., Barthel, M., Endt, K., Eberhard, M., Robbiani, R., Jacobi, C. A., Heikenwalder, M., Kirschning, C., Jung, S., Stallmach, T., Kremer, M., Hardt, W.-D. (2008). Microbe sampling by mucosal dendritic cells is a discrete, MyD88-independent stepin {Delta}invG S. Typhimurium colitis. JEM 205: 437-450 [Abstract] [Full Text]  
  • Raffatellu, M., Santos, R. L., Chessa, D., Wilson, R. P., Winter, S. E., Rossetti, C. A., Lawhon, S. D., Chu, H., Lau, T., Bevins, C. L., Adams, L. G., Baumler, A. J. (2007). The Capsule Encoding the viaB Locus Reduces Interleukin-17 Expression and Mucosal Innate Responses in the Bovine Intestinal Mucosa during Infection with Salmonella enterica Serotype Typhi. Infect. Immun. 75: 4342-4350 [Abstract] [Full Text]  
  • Vijay-Kumar, M., Wu, H., Jones, R., Grant, G., Babbin, B., King, T. P., Kelly, D., Gewirtz, A. T., Neish, A. S. (2006). Flagellin Suppresses Epithelial Apoptosis and Limits Disease during Enteric Infection. Am. J. Pathol. 169: 1686-1700 [Abstract] [Full Text]  
  • Stecher, B., Paesold, G., Barthel, M., Kremer, M., Jantsch, J., Stallmach, T., Heikenwalder, M., Hardt, W.-D. (2006). Chronic Salmonella enterica Serovar Typhimurium-Induced Colitis and Cholangitis in Streptomycin-Pretreated Nramp1+/+ Mice. Infect. Immun. 74: 5047-5057 [Abstract] [Full Text]  
  • Rollenhagen, C., Bumann, D. (2006). Salmonella enterica Highly Expressed Genes Are Disease Specific. Infect. Immun. 74: 1649-1660 [Abstract] [Full Text]  
  • Suar, M., Jantsch, J., Hapfelmeier, S., Kremer, M., Stallmach, T., Barrow, P. A., Hardt, W.-D. (2006). Virulence of Broad- and Narrow-Host-Range Salmonella enterica Serovars in the Streptomycin-Pretreated Mouse Model. Infect. Immun. 74: 632-644 [Abstract] [Full Text]  
  • Zhang, Y., Higashide, W., Dai, S., Sherman, D. M., Zhou, D. (2005). Recognition and Ubiquitination of Salmonella Type III Effector SopA by a Ubiquitin E3 Ligase, HsRMA1. J. Biol. Chem. 280: 38682-38688 [Abstract] [Full Text]  
  • Coombes, B. K., Coburn, B. A., Potter, A. A., Gomis, S., Mirakhur, K., Li, Y., Finlay, B. B. (2005). Analysis of the Contribution of Salmonella Pathogenicity Islands 1 and 2 to Enteric Disease Progression Using a Novel Bovine Ileal Loop Model and a Murine Model of Infectious Enterocolitis. Infect. Immun. 73: 7161-7169 [Abstract] [Full Text]  
  • Schlumberger, M. C., Muller, A. J., Ehrbar, K., Winnen, B., Duss, I., Stecher, B., Hardt, W.-D. (2005). Real-time imaging of type III secretion: Salmonella SipA injection into host cells. Proc. Natl. Acad. Sci. USA 102: 12548-12553 [Abstract] [Full Text]  
  • Coburn, B., Li, Y., Owen, D., Vallance, B. A., Finlay, B. B. (2005). Salmonella enterica Serovar Typhimurium Pathogenicity Island 2 Is Necessary for Complete Virulence in a Mouse Model of Infectious Enterocolitis. Infect. Immun. 73: 3219-3227 [Abstract] [Full Text]  
  • Stecher, B., Macpherson, A. J., Hapfelmeier, S., Kremer, M., Stallmach, T., Hardt, W.-D. (2005). Comparison of Salmonella enterica Serovar Typhimurium Colitis in Germfree Mice and Mice Pretreated with Streptomycin. Infect. Immun. 73: 3228-3241 [Abstract] [Full Text]  
  • Hapfelmeier, S., Stecher, B., Barthel, M., Kremer, M., Muller, A. J., Heikenwalder, M., Stallmach, T., Hensel, M., Pfeffer, K., Akira, S., Hardt, W.-D. (2005). The Salmonella Pathogenicity Island (SPI)-2 and SPI-1 Type III Secretion Systems Allow Salmonella Serovar typhimurium to Trigger Colitis via MyD88-Dependent and MyD88-Independent Mechanisms. J. Immunol. 174: 1675-1685 [Abstract] [Full Text]  
  • Brussow, H., Canchaya, C., Hardt, W.-D. (2004). Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion. Microbiol. Mol. Biol. Rev. 68: 560-602 [Abstract] [Full Text]  
  • Stecher, B., Hapfelmeier, S., Muller, C., Kremer, M., Stallmach, T., Hardt, W.-D. (2004). Flagella and Chemotaxis Are Required for Efficient Induction of Salmonella enterica Serovar Typhimurium Colitis in Streptomycin-Pretreated Mice. Infect. Immun. 72: 4138-4150 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»