This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by De Trez, C.
Right arrow Articles by Muraille, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by De Trez, C.
Right arrow Articles by Muraille, E.

 Previous Article  |  Next Article 

Infection and Immunity, February 2004, p. 824-832, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.824-832.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Myd88-Dependent In Vivo Maturation of Splenic Dendritic Cells Induced by Leishmania donovani and Other Leishmania Species

Carl De Trez,1 Maryse Brait,1 Oberdan Leo,1 Tony Aebischer,2 Fabiola Aguilar Torrentera,3 Yves Carlier,4* and Eric Muraille4*

Laboratory of Animal Physiology, Institut de Biologie et de Médecine Moléculaire (IBMM), Université Libre de Bruxelles, Gosselies,1 Laboratory of Parasitology, Université Libre de Bruxelles, Erasme, Belgium,4 Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany,2 Departamento of Inmunologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico Distrito Federal, Mexico3

Received 8 August 2003/ Returned for modification 16 September 2003/ Accepted 10 November 2003

The usual agent of visceral leishmaniasis in the Old World is Leishmania donovani, which typically produces systemic diseases in humans and mice. L. donovani has developed efficient strategies to infect and persist in macrophages from spleen and liver. Dendritic cells (DC) are sentinels of the immune system. Following recognition of evolutionary conserved microbial products, DC undergo a maturation process and activate antigen-specific naïve T cells. In the present report we provide new insights into how DC detect Leishmania in vivo. We demonstrate that in both C57BL/6 and BALB/c mice, systemic injection of L. donovani induced the migration of splenic DC from marginal zones to T-cell areas. During migration, DC upregulated the expression of major histocompatibility complex II and costimulatory receptors (such as CD40, CD80, and CD86). Leishmania-induced maturation requires live parasites and is not restricted to L. donovani, as L. braziliensis, L. major, and L. mexicana induced a similar process. Using a green fluorescent protein-expressing parasite, we demonstrate that DC undergoing maturation in vivo display no parasite internalization. We also show that L. donovani-induced DC maturation was partially abolished in MyD88-deficient mice. Taken together, our data suggest that Leishmania-induced DC maturation results from direct recognition of Leishmania by DC, and not from DC infection, and that MyD88-dependent receptors are implicated in this process.

* Corresponding author. Mailing address for Eric Muraille and Yves Carlier: Laboratory of Parasitology, Faculté de Médecine, Université Libre de Bruxelles, CP616, route de Lennik 808, Brussels B-1070, Belgium. Phone: 32 2 555 61 32. Fax: 32 2 555 61 28. E-mail for Eric Muraille: emuraille{at}hotmail.com. E-mail for Yves Carlier: ycarlier{at}ulb.ac.be.

Editor: W. A. Petri, Jr.

Infection and Immunity, February 2004, p. 824-832, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.824-832.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vargas-Inchaustegui, D. A., Tai, W., Xin, L., Hogg, A. E., Corry, D. B., Soong, L. (2009). Distinct Roles for MyD88 and Toll-Like Receptor 2 during Leishmania braziliensis Infection in Mice. Infect. Immun. 77: 2948-2956 [Abstract] [Full Text]  
  • Hkima Abou Fakher, F., Rachinel, N., Klimczak, M., Louis, J., Doyen, N. (2009). TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania major Favors Th1 Cell Development and the Resolution of Lesions. J. Immunol. 182: 1386-1396 [Abstract] [Full Text]  
  • Carvalho, L. P., Pearce, E. J., Scott, P. (2008). Functional Dichotomy of Dendritic Cells following Interaction with Leishmania braziliensis: Infected Cells Produce High Levels of TNF-{alpha}, whereas Bystander Dendritic Cells Are Activated to Promote T Cell Responses. J. Immunol. 181: 6473-6480 [Abstract] [Full Text]  
  • Tuon, F. F., Amato, V. S., Bacha, H. A., AlMusawi, T., Duarte, M. I., Amato Neto, V. (2008). Toll-Like Receptors and Leishmaniasis. Infect. Immun. 76: 866-872 [Full Text]  
  • Maroof, A., Kaye, P. M. (2008). Temporal Regulation of Interleukin-12p70 (IL-12p70) and IL-12-Related Cytokines in Splenic Dendritic Cell Subsets during Leishmania donovani Infection. Infect. Immun. 76: 239-249 [Abstract] [Full Text]  
  • Ato, M., Maroof, A., Zubairi, S., Nakano, H., Kakiuchi, T., Kaye, P. M. (2006). Loss of Dendritic Cell Migration and Impaired Resistance to Leishmania donovani Infection in Mice Deficient in CCL19 and CCL21. J. Immunol. 176: 5486-5493 [Abstract] [Full Text]  
  • Chen, X.-M., O'Hara, S. P., Nelson, J. B., Splinter, P. L., Small, A. J., Tietz, P. S., Limper, A. H., LaRusso, N. F. (2005). Multiple TLRs Are Expressed in Human Cholangiocytes and Mediate Host Epithelial Defense Responses to Cryptosporidium parvum via Activation of NF-{kappa}B. J. Immunol. 175: 7447-7456 [Abstract] [Full Text]  
  • Asselin-Paturel, C., Brizard, G., Chemin, K., Boonstra, A., O'Garra, A., Vicari, A., Trinchieri, G. (2005). Type I interferon dependence of plasmacytoid dendritic cell activation and migration. JEM 201: 1157-1167 [Abstract] [Full Text]  
  • Antoniazi, S., Price, H. P., Kropf, P., Freudenberg, M. A., Galanos, C., Smith, D. F., Muller, I. (2004). Chemokine Gene Expression in Toll-Like Receptor-Competent and -Deficient Mice Infected with Leishmania major. Infect. Immun. 72: 5168-5174 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»