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Infection and Immunity, February 2004, p. 833-843, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.833-843.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Candida albicans Yeast and Germ Tube Forms Interfere Differently with Human Monocyte Differentiation into Dendritic Cells: a Novel Dimorphism-Dependent Mechanism To Escape the Host's Immune Response

Antonella Torosantucci,1 Giulia Romagnoli,1 Paola Chiani,1 Annarita Stringaro,2 Pasqualina Crateri,2 Sabrina Mariotti,1 Raffaela Teloni,1 Giuseppe Arancia,2 Antonio Cassone,1 and Roberto Nisini1*

Laboratorio di Batteriologia e Micologia Medica,1 Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, 00161 Rome, Italy2

Received 14 May 2003/ Returned for modification 16 July 2003/ Accepted 31 October 2003

The ability of Candida albicans to convert from the yeast (Y) form to mycelial forms through germ tube (GT) formation is considered a key feature of the transition of the organism from commensalism to virulence. We show here that human monocytes cultured with granulocyte-macrophage colony-stimulating factor and interleukin-4 (IL-4) after phagocytosis of Y forms did not differentiate into dendritic cells (DCs); they retained CD14, did not acquire CD1a, and were unable to express the maturation markers CD83 and CCR7. Moreover, they did not produce IL-12p70 but secreted IL-10. In addition, they spontaneously expressed high levels of tumor necrosis factor alpha (TNF-{alpha}), IL-6, and IL-8 mRNA transcripts and were able to induce proliferation of alloreactive memory but not naïve T lymphocytes. Conversely, monocytes that had phagocytosed GT forms differentiated into mature CD83+ and CCR7+ DCs; however, there was no up-regulation of CD40, CD80, and major histocompatibility complex class II, irrespective of lipopolysaccharide (LPS) treatment. In addition, these cells were unable to produce IL-12 even after LPS stimulation, but they were not functionally exhausted, as shown by their capacity to express TNF-{alpha} and IL-8 mRNA transcripts. These cells were able to prime naïve T cells but not to induce their functional polarization into effector cells. These data indicate that phagocytosis of Y and GT forms has profound and distinct effects on the differentiation pathway of monocytes. Thus, the differentiation of human monocytes into DCs appears to be tunable and exploitable by C. albicans to elude immune surveillance.

* Corresponding author. Mailing address: Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39 06 4990 2659. Fax: 39 06 49387112. E-mail: r.nisini{at}iss.it.

Editor: S. H. E. Kaufmann

Infection and Immunity, February 2004, p. 833-843, Vol. 72, No. 2
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.2.833-843.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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