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Infection and Immunity, March 2004, p. 1496-1503, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1496-1503.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evidence for a Hybrid Genomic Island in Verocytotoxin-Producing Escherichia coli CL3 (Serotype O113:H21) Containing Segments of EDL933 (Serotype O157:H7) O Islands 122 and 48

Songhai Shen,1 Mariola Mascarenhas,1 Kris Rahn,1 James B. Kaper,2 and Mohamed A. Karmal1,3*

Laboratory for Foodborne Zoonoses, Population and Public Health Branch, Health Canada, Guelph, Ontario N1G 3W4, Canada,1 Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201,2 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada3

Received 9 June 2003/ Returned for modification 18 August 2003/ Accepted 13 November 2003

Genomic O island 122 (OI-122) of the verocytotoxin-producing Escherichia coli (VTEC) strain EDL933 contains four putative virulence genes, Z4321, Z4326, Z4332, and Z4333. However, strain CL3 (serotype O113:H21) contains only Z4321, not the other three genes. To determine whether Z4321 is part of a different genomic island in CL3, a region of 27,293 bp up- and downstream of Z4321 was sequenced and found to contain elements of two different EDL933 genomic islands (OI-48 and OI-122) and a Yersinia pestis-like hemolysin/adhesin gene cluster. The region contained OI-48 genes Z1635, Z1636, and Z1637 at the left terminus and Z1641, Z1642, Z1643, and Z1644 at the right. The middle portion consisted of OI-48 gene Z1640, which was separated into three fragments by genomic segments including the Y. pestis cluster and EDL933 OI-122 genes Z4322, Z4321, and Z4318. In a PCR investigation of 36 VTEC strains of different serotypes, intact Z1640 was present in strains of serotypes O157:H7, O26:H11, O103:H2, O111:NM, and O145:NM, which are associated with hemolytic uremic syndrome and outbreaks. In contrast, fragmented Z1640 was seen in strains of nonepidemic serotypes, such as O91:H21 and O113:H21, and in animal serotypes that have not been associated with human disease, indicating that Z1640 might be a virulence gene.

* Corresponding author. Mailing address: Laboratory for Foodborne Zoonoses, Population and Public Health Branch, Health Canada, 110 Stone Road West, Guelph, Ontario, Canada, N1G 3W4. Phone: 519 822 3300. Fax: 519 822 2280. E-mail: Mohamed_Karmali{at}hc-sc.gc.ca.

Editor: B. B. Finlay

Infection and Immunity, March 2004, p. 1496-1503, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1496-1503.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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