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Infection and Immunity, March 2004, p. 1530-1536, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1530-1536.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Populations of Human T Lymphocytes That Traverse the Vascular Endothelium Stimulated by Borrelia burgdorferi Are Enriched with Cells That Secrete Gamma Interferon

Edna I. Gergel and Martha B. Furie*

Center for Infectious Diseases and Department of Pathology, Stony Brook University, Stony Brook, New York 11794-5120

Received 25 August 2003/ Returned for modification 8 October 2003/ Accepted 11 December 2003

Some diseases are characterized by prevalence in the affected tissues of type 1 T lymphocytes, which secrete gamma interferon (IFN-{gamma}) and other proinflammatory cytokines. For example, type 1 T cells predominate in the lesions of patients with Lyme disease, which is caused by the bacterium Borrelia burgdorferi. We used an in vitro model of the blood vessel wall to test the premise that the vascular endothelium actively recruits circulating type 1 T cells to such lesions. When T lymphocytes isolated from human peripheral blood were examined, the populations that traversed monolayers of resting human umbilical vein endothelial cells (HUVEC) or HUVEC stimulated by interleukin-1ß or B. burgdorferi were markedly enriched for T cells that produced IFN-{gamma} compared to the initially added population of T cells. No enrichment was seen for cells that produced interleukin-4, a marker for type 2 T lymphocytes. Very late antigen-4 and CD11/CD18 integrins mediated passage of the T cells across both resting and stimulated HUVEC, and the endothelium-derived chemokine CCL2 (monocyte chemoattractant protein 1) was responsible for the enhanced migration of T cells across stimulated HUVEC. These results suggest that the vascular endothelium may contribute to the selective accumulation of type 1 T cells in certain pathological lesions, including those of Lyme disease.

* Corresponding author. Mailing address: Center for Infectious Diseases, Room 240, Centers for Molecular Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-5120. Phone: (631) 632-4232. Fax: (631) 632-4294. E-mail: mfurie{at}notes.cc.sunysb.edu.

Editor: D. L. Burns

Infection and Immunity, March 2004, p. 1530-1536, Vol. 72, No. 3
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.3.1530-1536.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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