This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holm, M. M.
Right arrow Articles by Lafontaine, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holm, M. M.
Right arrow Articles by Lafontaine, E. R.

 Previous Article  |  Next Article 

Infection and Immunity, April 2004, p. 1906-1913, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.1906-1913.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Moraxella catarrhalis Porin-Like Outer Membrane Protein CD Is an Adhesin for Human Lung Cells

Melissa M. Holm, Serena L. Vanlerberg, Ian M. Foley, Darren D. Sledjeski, and Eric R. Lafontaine*

Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 43614-5806

Received 4 August 2003/ Returned for modification 4 November 2003/ Accepted 27 December 2003

The outer membrane protein CD (OMPCD) of Moraxella catarrhalis is an outer membrane protein with several attributes of a potential vaccine antigen. We isolated four transposon mutants of strain O35E on the basis of their reduced binding to A549 human lung cells in microcolony formation assays, and we determined that they contain a transposon in ompCD. We also found that these transposon insertions had pleiotropic effects: mutants grew slower, became serum sensitive, bound ~10-fold less to A549 cells, and appeared transparent when grown on solid medium. We confirmed that these various phenotypes could be attributed solely to disruption of ompCD by constructing the isogenic strain O35E.CD1. O35E-ompCD was cloned, and recombinant Escherichia coli bacteria expressing the gene product exhibited a 10-fold increase in adherence to A549 cells. This is the first report of M. catarrhalis ompCD mutants, and our findings demonstrate that this gene product is an adhesin for human lung cells.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College of Ohio, Health Education Building, 3055 Arlington Ave., Toledo, Ohio 43614-5806. Phone: (419) 383-6626. Fax: (419) 383-3002. E-mail: elafontaine{at}mco.edu.

Editor: V. J. DiRita

Infection and Immunity, April 2004, p. 1906-1913, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.1906-1913.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Balder, R., Krunkosky, T. M., Nguyen, C. Q., Feezel, L., Lafontaine, E. R. (2009). Hag Mediates Adherence of Moraxella catarrhalis to Ciliated Human Airway Cells. Infect. Immun. 77: 4597-4608 [Abstract] [Full Text]  
  • de Vries, S. P. W., Bootsma, H. J., Hays, J. P., Hermans, P. W. M. (2009). Molecular Aspects of Moraxella catarrhalis Pathogenesis. Microbiol. Mol. Biol. Rev. 73: 389-406 [Abstract] [Full Text]  
  • Wang, W., Richardson, A. R., Martens-Habbena, W., Stahl, D. A., Fang, F. C., Hansen, E. J. (2008). Identification of a Repressor of a Truncated Denitrification Pathway in Moraxella catarrhalis. J. Bacteriol. 190: 7762-7772 [Abstract] [Full Text]  
  • Brooks, M. J., Sedillo, J. L., Wagner, N., Laurence, C. A., Wang, W., Attia, A. S., Hansen, E. J., Gray-Owen, S. D. (2008). Modular Arrangement of Allelic Variants Explains the Divergence in Moraxella catarrhalis UspA Protein Function. Infect. Immun. 76: 5330-5340 [Abstract] [Full Text]  
  • Brooks, M. J., Sedillo, J. L., Wagner, N., Wang, W., Attia, A. S., Wong, H., Laurence, C. A., Hansen, E. J., Gray-Owen, S. D. (2008). Moraxella catarrhalis Binding to Host Cellular Receptors Is Mediated by Sequence-Specific Determinants Not Conserved among All UspA1 Protein Variants. Infect. Immun. 76: 5322-5329 [Abstract] [Full Text]  
  • Luke, N. R., Jurcisek, J. A., Bakaletz, L. O., Campagnari, A. A. (2007). Contribution of Moraxella catarrhalis Type IV Pili to Nasopharyngeal Colonization and Biofilm Formation. Infect. Immun. 75: 5559-5564 [Abstract] [Full Text]  
  • Wang, W., Reitzer, L., Rasko, D. A., Pearson, M. M., Blick, R. J., Laurence, C., Hansen, E. J. (2007). Metabolic Analysis of Moraxella catarrhalis and the Effect of Selected In Vitro Growth Conditions on Global Gene Expression. Infect. Immun. 75: 4959-4971 [Abstract] [Full Text]  
  • Pearson, M. M., Hansen, E. J. (2007). Identification of Gene Products Involved in Biofilm Production by Moraxella catarrhalis ETSU-9 In Vitro. Infect. Immun. 75: 4316-4325 [Abstract] [Full Text]  
  • Balder, R., Hassel, J., Lipski, S., Lafontaine, E. R. (2007). Moraxella catarrhalis Strain O35E Expresses Two Filamentous Hemagglutinin-Like Proteins That Mediate Adherence to Human Epithelial Cells. Infect. Immun. 75: 2765-2775 [Abstract] [Full Text]  
  • Liu, D.-F., McMichael, J. C., Baker, S. M. (2007). Moraxella catarrhalis Outer Membrane Protein CD Elicits Antibodies That Inhibit CD Binding to Human Mucin and Enhance Pulmonary Clearance of M. catarrhalis in a Mouse Model. Infect. Immun. 75: 2818-2825 [Abstract] [Full Text]  
  • Becker, P. D., Bertot, G. M., Souss, D., Ebensen, T., Guzman, C. A., Grinstein, S. (2007). Intranasal Vaccination with Recombinant Outer Membrane Protein CD and Adamantylamide Dipeptide as the Mucosal Adjuvant Enhances Pulmonary Clearance of Moraxella catarrhalis in an Experimental Murine Model. Infect. Immun. 75: 1778-1784 [Abstract] [Full Text]  
  • Brooks, M. J., Laurence, C. A., Hansen, E. J., Gray-Owen, S. D. (2007). Characterization of the Moraxella catarrhalis Opa-Like Protein, OlpA, Reveals a Phylogenetically Conserved Family of Outer Membrane Proteins. J. Bacteriol. 189: 76-82 [Abstract] [Full Text]  
  • Lipski, S. L., Akimana, C., Timpe, J. M., Wooten, R. M., Lafontaine, E. R. (2007). The Moraxella catarrhalis Autotransporter McaP Is a Conserved Surface Protein That Mediates Adherence to Human Epithelial Cells through Its N-Terminal Passenger Domain. Infect. Immun. 75: 314-324 [Abstract] [Full Text]  
  • Pearson, M. M., Laurence, C. A., Guinn, S. E., Hansen, E. J. (2006). Biofilm Formation by Moraxella catarrhalis In Vitro: Roles of the UspA1 Adhesin and the Hag Hemagglutinin. Infect. Immun. 74: 1588-1596 [Abstract] [Full Text]  
  • Attia, A. S., Ram, S., Rice, P. A., Hansen, E. J. (2006). Binding of Vitronectin by the Moraxella catarrhalis UspA2 Protein Interferes with Late Stages of the Complement Cascade. Infect. Immun. 74: 1597-1611 [Abstract] [Full Text]  
  • Hays, J. P., van Selm, S., Hoogenboezem, T., Estevao, S., Eadie, K., van Veelen, P., Tommassen, J., van Belkum, A., Hermans, P. W. M. (2005). Identification and Characterization of a Novel Outer Membrane Protein (OMP J) of Moraxella catarrhalis That Exists in Two Major Forms. J. Bacteriol. 187: 7977-7984 [Abstract] [Full Text]  
  • Edwards, K. J., Schwingel, J. M., Datta, A. K., Campagnari, A. A. (2005). Multiplex PCR Assay That Identifies the Major Lipooligosaccharide Serotype Expressed by Moraxella catarrhalis Clinical Isolates. J. Clin. Microbiol. 43: 6139-6143 [Abstract] [Full Text]  
  • Easton, D. M., Smith, A., Gallego, S. G., Foxwell, A. R., Cripps, A. W., Kyd, J. M. (2005). Characterization of a Novel Porin Protein from Moraxella catarrhalis and Identification of an Immunodominant Surface Loop. J. Bacteriol. 187: 6528-6535 [Abstract] [Full Text]  
  • Nordstrom, T., Blom, A. M., Tan, T. T., Forsgren, A., Riesbeck, K. (2005). Ionic Binding of C3 to the Human Pathogen Moraxella catarrhalis Is a Unique Mechanism for Combating Innate Immunity. J. Immunol. 175: 3628-3636 [Abstract] [Full Text]  
  • Bullard, B., Lipski, S. L., Lafontaine, E. R. (2005). Hag Directly Mediates the Adherence of Moraxella catarrhalis to Human Middle Ear Cells. Infect. Immun. 73: 5127-5136 [Abstract] [Full Text]  
  • Peng, D., Choudhury, B. P., Petralia, R. S., Carlson, R. W., Gu, X.-X. (2005). Roles of 3-Deoxy-D-manno-2-Octulosonic Acid Transferase from Moraxella catarrhalis in Lipooligosaccharide Biosynthesis and Virulence. Infect. Immun. 73: 4222-4230 [Abstract] [Full Text]  
  • Yu, S., Gu, X.-X. (2005). Synthesis and Characterization of Lipooligosaccharide-Based Conjugate Vaccines for Serotype B Moraxella catarrhalis. Infect. Immun. 73: 2790-2796 [Abstract] [Full Text]  
  • Edwards, K. J., Allen, S., Gibson, B. W., Campagnari, A. A. (2005). Characterization of a Cluster of Three Glycosyltransferase Enzymes Essential for Moraxella catarrhalis Lipooligosaccharide Assembly. J. Bacteriol. 187: 2939-2947 [Abstract] [Full Text]  
  • Attia, A. S., Lafontaine, E. R., Latimer, J. L., Aebi, C., Syrogiannopoulos, G. A., Hansen, E. J. (2005). The UspA2 Protein of Moraxella catarrhalis Is Directly Involved in the Expression of Serum Resistance. Infect. Immun. 73: 2400-2410 [Abstract] [Full Text]  
  • Lafontaine, E. R., Wall, D., Vanlerberg, S. L., Donabedian, H., Sledjeski, D. D. (2004). Moraxella catarrhalis Coaggregates with Streptococcus pyogenes and Modulates Interactions of S. pyogenes with Human Epithelial Cells. Infect. Immun. 72: 6689-6693 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»