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Infection and Immunity, April 2004, p. 2152-2159, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2152-2159.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Expression of Chemokines and Proinflammatory Cytokines in Newly Hatched Chickens Infected with Salmonella enterica Serovar Typhimurium

G. S. K. Withanage,1* Pete Kaiser,2 Paul Wigley,2 Claire Powers,2 Pietro Mastroeni,1 Heather Brooks,1 Paul Barrow,2 Adrian Smith,2 Duncan Maskell,1 and Ian McConnell1

Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES,1 Institute for Animal Health, Compton, Berkshire RG20 7NN, United Kingdom2

Received 11 November 2003/ Returned for modification 2 December 2003/ Accepted 13 January 2004

Poultry meat and eggs contaminated with Salmonella enterica serovar Enteritidis or Salmonella enterica serovar Typhimurium are common sources of acute gastroenteritis in humans. However, the exact nature of the immune mechanisms protective against Salmonella infection in chickens has not been characterized at the molecular level. In the present study, bacterial colonization, development of pathological lesions, and proinflammatory cytokine and chemokine gene expression were investigated in the liver, spleen, jejunum, ileum, and cecal tonsils in newly hatched chickens 6, 12, 24, and 48 h after oral infection with Salmonella serovar Typhimurium. Very high bacterial counts were found in the ileum and cecal contents throughout the experiment, whereas Salmonella started to appear in the liver only from 24 h postinfection. Large numbers of heterophils, equivalent to neutrophils in mammals, and inflammatory edema could be seen in the lamina propria of the intestinal villi and in the liver. Interleukin 8 (IL-8), K60 (a CXC chemokine), macrophage inflammatory protein 1 ß, and IL-1ß levels were significantly upregulated in the intestinal tissues and in the livers of the infected birds. However, the spleens of the infected birds show little or no change in the expression levels of these cytokines and chemokines. Increased expression of the proinflammatory cytokines and chemokines (up to several hundred-fold) correlated with the presence of inflammatory signs in those tissues. This is the first description of in vivo expression of chemokines and proinflammatory cytokines in response to oral infection with Salmonella in newly hatched chickens.

* Corresponding author. Mailing address: Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom. Phone: 44 1223 765801. Fax: 44-1223-765802. E-mail: gskw2{at}cam.ac.uk.

Editor: F. C. Fang

Infection and Immunity, April 2004, p. 2152-2159, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2152-2159.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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