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Infection and Immunity, April 2004, p. 2160-2169, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2160-2169.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cell Surface-Associated Elongation Factor Tu Mediates the Attachment of Lactobacillus johnsonii NCC533 (La1) to Human Intestinal Cells and Mucins

Dominique Granato,* Gabriela E. Bergonzelli, Raymond David Pridmore, Laure Marvin, Martine Rouvet, and Irène E. Corthésy-Theulaz

Nestlé Research Center, CH-1000 Lausanne 26, Switzerland

Received 2 October 2003/ Returned for modification 28 October 2003/ Accepted 8 January 2004

The aim of this work was to identify Lactobacillus johnsonii NCC533 (La1) surface molecules mediating attachment to intestinal epithelial cells and mucins. Incubation of Caco-2 intestinal epithelial cells with an L. johnsonii La1 cell wall extract led to the recognition of elongation factor Tu (EF-Tu) as a novel La1 adhesin-like factor. The presence of EF-Tu at the surface of La1 was confirmed by analysis of purified outer surface protein extract by immunoblotting experiments, by electron microscopy, and by enzyme-linked immunosorbent assays of live bacteria. Furthermore, tandem mass spectrometry analysis proved that EF-TU was expressed at the La1 surface as an intact molecule. Using recombinant La1 EF-Tu protein, we were able to determine that its binding to intestinal cells and to mucins is pH dependent. Competition experiments suggested that EF-Tu has an important role in La1 mucin binding capacity. In addition, immunomodulation studies performed on HT29 cells showed that EF-Tu recombinant protein can induce a proinflammatory response in the presence of soluble CD14. Our in vitro results indicate that EF-Tu, through its binding to the intestinal mucosa, might participate in gut homeostasis.

* Corresponding author. Mailing address: Nestlé Research Center, CH-1000 Lausanne 26, Switzerland. Phone: 41 21 785 86 85. Fax: 41 21 785 85 44. E-mail: dominique-anne.granato{at}rdls.nestle.com.

Editor: F. C. Fang

Infection and Immunity, April 2004, p. 2160-2169, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2160-2169.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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