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SKN7 of Candida albicans: Mutant Construction and Phenotype Analysis

Department of Microbiology and Immunology, Georgetown University Medical Center,¹ Department of Biological and Environmental Sciences, The University of the District of Columbia, Washington, D.C.²

Received 22 November 2003/ Returned for modification 17 December 2003/ Accepted 22 December 2003

The SKN7 two-component response regulator gene of Candida albicans was deleted, and the phenotype of the mutant was established. This mutant exhibited impaired growth on Spider agar and 10% serum agar compared to wild-type and gene-reconstituted strains. The skn7 mutant was sensitive to H₂O₂ in vitro, but its virulence was only mildly attenuated. A comparison of the Skn7p and Ssk1p response regulators of C. albicans is discussed.

Editor: T. R. Kozel

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