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Infection and Immunity, April 2004, p. 2390-2394, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2390-2394.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SKN7 of Candida albicans: Mutant Construction and Phenotype Analysis

Praveen Singh,1 Neeraj Chauhan,1 Anup Ghosh,1 Freddie Dixon,2 and Richard Calderone1*

Department of Microbiology and Immunology, Georgetown University Medical Center,1 Department of Biological and Environmental Sciences, The University of the District of Columbia, Washington, D.C.2

Received 22 November 2003/ Returned for modification 17 December 2003/ Accepted 22 December 2003

The SKN7 two-component response regulator gene of Candida albicans was deleted, and the phenotype of the mutant was established. This mutant exhibited impaired growth on Spider agar and 10% serum agar compared to wild-type and gene-reconstituted strains. The skn7 mutant was sensitive to H2O2 in vitro, but its virulence was only mildly attenuated. A comparison of the Skn7p and Ssk1p response regulators of C. albicans is discussed.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Georgetown University Medical Center, SE 312 Med Dent Building, 3900 Reservoir Rd. NW, Washington, DC 20007. Phone: (202) 687-1137. Fax: (202) 687-1800. E-mail: calderor{at}georgetown.edu.

Editor: T. R. Kozel

Infection and Immunity, April 2004, p. 2390-2394, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2390-2394.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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