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Infection and Immunity, April 2004, p. 2445-2448, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2445-2448.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Enterococcal Aggregation Substance and Binding Substance Are Not Major Contributors to Urinary Tract Colonization by Enterococcus faecalis in a Mouse Model of Ascending Unobstructed Urinary Tract Infection

James R. Johnson,1,2* Connie Clabots,1,2 Helmut Hirt,3,{dagger} Christopher Waters,3,{ddagger} and Gary Dunny3

Mucosal and Vaccine Research Center, VA Medical Center,1 Departments of Medicine,2 Microbiology, University of Minnesota, Minneapolis, Minnesota3

Received 12 September 2003/ Returned for modification 12 November 2003/ Accepted 5 January 2004

Isogenic Enterococcus faecalis strains that differ in their expression of aggregation substance (AS) and its cognate receptor, enterococcal binding substance (EBS), were compared for urovirulence in mice. Strain OG1SSp/pCF500 (inducible AS+, constitutive EBS+) failed to outcompete isogenic derivative INY3000 (AS- EBS-) in the urine, bladders, or kidneys of mice harvested at 48 h postinoculation. Neither mouse nor human urine induced AS expression by OG1SSp/pCF500. Recombinant strain OG1SSp/pINY1801 (constitutive AS+, EBS+) exhibited plasmid segregation that was as extensive in vivo as in vitro. These data suggest that AS and EBS do not contribute to upper or lower urinary tract colonization by E. faecalis and that growth in urine does not induce AS expression by strains carrying plasmids in the pCF10 family.

* Corresponding author. Mailing address: Infectious Diseases (111F), Minneapolis VA Medical Center, One Veterans Dr., Minneapolis, MN 55417. Phone: (612) 467-4185. Fax: (612) 727-5995. E-mail: johns007{at}umn.edu.

Editor: B. B. Finlay

{dagger} Present address: Department of Biology, Kansas State University, Manhattan, Kans.

{ddagger} Present address: Department of Molecular Biology, Princeton University, Princeton, N.J.

Infection and Immunity, April 2004, p. 2445-2448, Vol. 72, No. 4
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.4.2445-2448.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kau, A. L., Martin, S. M., Lyon, W., Hayes, E., Caparon, M. G., Hultgren, S. J. (2005). Enterococcus faecalis Tropism for the Kidneys in the Urinary Tract of C57BL/6J Mice. Infect. Immun. 73: 2461-2468 [Abstract] [Full Text]  


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