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Infection and Immunity, May 2004, p. 2462-2467, Vol. 72, No. 5
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.5.2462-2467.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Association of Tuberculin-Boosted Antibody Responses with Pathology and Cell-Mediated Immunity in Cattle Vaccinated with Mycobacterium bovis BCG and Infected with M. bovis

Konstantin Lyashchenko,1 Adam O. Whelan,2 Rena Greenwald,1 John M. Pollock,3 Peter Andersen,4 R. Glyn Hewinson,2 and H. Martin Vordermeier2*

Chembio Diagnostic Systems, Inc., Medford, New York,1 Veterinary Laboratories Agency Weybridge, TB Research Group, New Haw, Addlestone, Surrey,2 Veterinary Science Division, Department of Agriculture and Rural Affairs of Northern Ireland, Stormont, Belfast, United Kingdom,3 Statens Serum Institut, Department of TB Immunlogy, Copenhagen, Denmark4

Received 4 September 2003/ Returned for modification 18 December 2003/ Accepted 16 January 2004

Vaccine development and our understanding of the pathology of bovine tuberculosis in cattle would be greatly facilitated by definition of the immunological correlates of protection and/or pathology. In this study we analyzed humoral immune responses in Mycobacterium bovis BCG-vaccinated and control cattle (in particular, the relationship between the intradermal comparative tuberculin skin test and serum immunoglobulin G [IgG] responses) against a range of mycobacterial antigens (MPB59, MPB64, MPB70, MPB83, ESAT-6, CFP-10, Acr1, and PstS-1) by multiantigen print immunoassay and conventional enzyme-linked immunosorbent assay. Following M. bovis infection, the comparative tuberculin skin test strongly boosted IgG, IgG1, and IgG2 antibody responses, particularly against MPB83 and MPB70, in unvaccinated cattle but failed to boost these responses, or did so only weakly, in BCG-vaccinated calves. In addition, the skin test-induced increases in MPB83-specific IgG responses correlated positively with bacterial loads and ESAT-6-induced in vitro gamma interferon responses. In conclusion, both the negative correlation of skin test-enhanced MPB83-specific antibody responses with BCG-induced protection and their positive correlation with bacterial loads can serve as useful markers for vaccine efficacy after challenge.

* Corresponding author. Mailing address: VLA Weybridge, TB Research Group, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 44 1932 357 884. Fax: 44 1032 357 684. E-mail: m.vordermeier{at}vla.defra.gsi.gov.uk.

Editor: A. D. O'Brien

Infection and Immunity, May 2004, p. 2462-2467, Vol. 72, No. 5
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.5.2462-2467.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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