This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Young, V. B. Articles by Schauer, D. B. Search for Related Content PubMed PubMed Citation Articles by Young, V. B. Articles by Schauer, D. B.

 Previous Article  |  Next Article 

Infection and Immunity, May 2004, p. 2521-2527, Vol. 72, No. 5
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.5.2521-2527.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

In Vitro and In Vivo Characterization of Helicobacter hepaticus Cytolethal Distending Toxin Mutants

Department of Microbiology and Molecular Genetics,¹ Infectious Diseases Unit, Department of Internal Medicine,² National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824,³ Division of Comparative Medicine,⁴ Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139⁵

Received 16 December 2003/ Returned for modification 16 January 2004/ Accepted 21 January 2004

Helicobacter hepaticus expresses a member of the cytolethal distending toxin (CDT) family of bacterial cytotoxins. To investigate the role of CDT in the pathogenesis of H. hepaticus, transposon mutagenesis was used to generate a series of isogenic mutants in and around the cdtABC gene cluster. An H. hepaticus transposon mutant with a disrupted cdtABC coding region no longer produced CDT activity. Conversely, a transposon insertion outside of the cluster did not affect the CDT activity. An examination of these mutants demonstrated that CDT represents the previously described granulating cytotoxin in H. hepaticus. Challenge of C57BL/6 interleukin 10^–/– mice with isogenic H. hepaticus mutants revealed that CDT expression is not required for colonization of the murine gut. However, a CDT-negative H. hepaticus mutant had a significantly diminished capacity to induce lesions in this murine model of inflammatory bowel disease.

Editor: D. L. Burns

This article has been cited by other articles:

• Krishnan, N., Doster, A. R., Duhamel, G. E., Becker, D. F. (2008). Characterization of a Helicobacter hepaticus putA Mutant Strain in Host Colonization and Oxidative Stress. Infect. Immun. 76: 3037-3044 [Abstract] [Full Text]  
• Sterzenbach, T., Lee, S. K., Brenneke, B., von Goetz, F., Schauer, D. B., Fox, J. G., Suerbaum, S., Josenhans, C. (2007). Inhibitory Effect of Enterohepatic Helicobacter hepaticus on Innate Immune Responses of Mouse Intestinal Epithelial Cells. Infect. Immun. 75: 2717-2728 [Abstract] [Full Text]  
• Hong, Y., Wang, G., Maier, R. J. (2007). A Helicobacter hepaticus catalase mutant is hypersensitive to oxidative stress and suffers increased DNA damage. J Med Microbiol 56: 557-562 [Abstract] [Full Text]  
• Mansfield, L. S., Bell, J. A., Wilson, D. L., Murphy, A. J., Elsheikha, H. M., Rathinam, V. A. K., Fierro, B. R., Linz, J. E., Young, V. B. (2007). C57BL/6 and Congenic Interleukin-10-Deficient Mice Can Serve as Models of Campylobacter jejuni Colonization and Enteritis. Infect. Immun. 75: 1099-1115 [Abstract] [Full Text]  
• Hue, S., Ahern, P., Buonocore, S., Kullberg, M. C., Cua, D. J., McKenzie, B. S., Powrie, F., Maloy, K. J. (2006). Interleukin-23 drives innate and T cell-mediated intestinal inflammation. J. Exp. Med. 203: 2473-2483 [Abstract] [Full Text]  
• Pratt, J. S., Sachen, K. L., Wood, H. D., Eaton, K. A., Young, V. B. (2006). Modulation of Host Immune Responses by the Cytolethal Distending Toxin of Helicobacter hepaticus.. Infect. Immun. 74: 4496-4504 [Abstract] [Full Text]  
• Kusters, J. G., van Vliet, A. H. M., Kuipers, E. J. (2006). Pathogenesis of Helicobacter pylori Infection. Clin. Microbiol. Rev. 19: 449-490 [Abstract] [Full Text]  
• Kuehl, C. J., Wood, H. D., Marsh, T. L., Schmidt, T. M., Young, V. B. (2005). Colonization of the Cecal Mucosa by Helicobacter hepaticus Impacts the Diversity of the Indigenous Microbiota. Infect. Immun. 73: 6952-6961 [Abstract] [Full Text]  
• Mehta, N. S., Benoit, S., Mysore, J. V., Sousa, R. S., Maier, R. J. (2005). Helicobacter hepaticus Hydrogenase Mutants Are Deficient in Hydrogen-Supported Amino Acid Uptake and in Causing Liver Lesions in A/J Mice. Infect. Immun. 73: 5311-5318 [Abstract] [Full Text]  
• Ge, Z., Feng, Y., Whary, M. T., Nambiar, P. R., Xu, S., Ng, V., Taylor, N. S., Fox, J. G. (2005). Cytolethal Distending Toxin Is Essential for Helicobacter hepaticus Colonization in Outbred Swiss Webster Mice. Infect. Immun. 73: 3559-3567 [Abstract] [Full Text]  
• Heywood, W., Henderson, B., Nair, S. P (2005). Cytolethal distending toxin: creating a gap in the cell cycle. J Med Microbiol 54: 207-216 [Abstract] [Full Text]  
• Hyma, K. E., Lacher, D. W., Nelson, A. M., Bumbaugh, A. C., Janda, J. M., Strockbine, N. A., Young, V. B., Whittam, T. S. (2005). Evolutionary Genetics of a New Pathogenic Escherichia Species: Escherichia albertii and Related Shigella boydii Strains. J. Bacteriol. 187: 619-628 [Abstract] [Full Text]  
• Rogers, A. B., Boutin, S. R., Whary, M. T., Sundina, N., Zhongming Ge, , Cormier, K., Fox, J. G. (2004). Progression of Chronic Hepatitis and Preneoplasia in Helicobacter hepaticus-Infected A/JCr Mice. Toxicol Pathol 32: 668-677 [Abstract]  
• Boutin, S. R., Rogers, A. B., Zeli Shen, , Fry, R. C., Love, J. A., Nambiar, P. R., Suerbaum, S., Fox, J. G. (2004). Hepatic Temporal Gene Expression Profiling in Helicobacter hepaticus-Infected A/JCr Mice. Toxicol Pathol 32: 678-693 [Abstract]