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Infection and Immunity, May 2004, p. 2837-2842, Vol. 72, No. 5
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.5.2837-2842.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

pagP Is Required for Resistance to Antibody-Mediated Complement Lysis during Bordetella bronchiseptica Respiratory Infection

Mylisa R. Pilione,1,2 Elizabeth J. Pishko,1 Andrew Preston,3 Duncan J. Maskell,4 and Eric T. Harvill1,2*

Department of Veterinary Science,1 Graduate Program in Biochemistry, Microbiology and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania,2 Department of Microbiology, The University of Guelph, Guelph, Ontario, Canada,3 Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom4

Received 9 December 2003/ Returned for modification 30 December 2003/ Accepted 28 January 2004

To efficiently colonize and persist in the lower respiratory tract, bacteria must survive multiple host immune mechanisms. Bordetella bronchiseptica is a gram-negative respiratory pathogen that naturally infects mice and persists in the lower respiratory tract for up to 49 days postinoculation. In this work, we examined the effect of mutation of the pagP gene on the persistence of B. bronchiseptica in the lower respiratory tract of mice. The pagP gene encodes a palmitoyl transferase that is responsible for the addition of a palmitoyl group to the lipid A region of B. bronchiseptica lipopolysaccharide. Data presented here confirm that a B. bronchiseptica {Delta}pagP mutant demonstrates defective persistence in the lower respiratory tract of wild-type mice. We hypothesized that the defective persistence of the B. bronchiseptica {Delta}pagP mutant was due to an increased susceptibility of this mutant to a host immune response. In vivo data indicate that both B cells and the complement component C3 are required for the reduced bacterial numbers of the {Delta}pagP mutant on day 14 postinoculation. In addition, an in vitro complement killing assay demonstrated that B. bronchiseptica exhibits pagP-dependent resistance to antibody-mediated complement killing at low concentrations of immune serum. Taken together, these results suggest that pagP is required for B. bronchiseptica to resist antibody-mediated complement lysis during respiratory infection.

* Corresponding author. Mailing address: The Pennsylvania State University, Department of Veterinary Science, University Park, PA 16802. Phone: (814) 863-8522. Fax: (814) 863-6140. E-mail: eth10{at}psu.edu.

Editor: D. L. Burns

Infection and Immunity, May 2004, p. 2837-2842, Vol. 72, No. 5
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.5.2837-2842.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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