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Infection and Immunity, June 2004, p. 3106-3112, Vol. 72, No. 6
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.6.3106-3112.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus cereus with Fully Differentiated Human Intestinal Caco-2 Cells

Jessica Minnaard,1 Vanessa Lievin-Le Moal,2 Marie-Helene Coconnier,2 Alain L. Servin,2* and Pablo F. Pérez1

Centro de Investigación y Desarrollo en Criotecnología de Alimentos, Cátedra de Microbiología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata 1900, Argentina,1 Institut National de la Santé et de la Recherche Médicale, Unité 510, Faculté de Pharmacie Paris XI, F-92296 ChÂtenay-Malabry, France2

Received 12 May 2003/ Returned for modification 10 July 2003/ Accepted 19 January 2004

In the present study, the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully differentiated Caco-2 cells with B. cereus in the exponential phase of growth, in order to minimize the concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures. Infection of enterocyte-like cells with B. cereus leads to disruption of the F-actin network and necrosis. Even though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be necessary. In addition, we observed that some B. cereus strains were able to be internalized in Caco-2 cells. Our findings add a new insight into the mechanisms of virulence of B. cereus in the context of intestinal infection.

* Corresponding author. Mailing address: Institut National de la Santé et de la Recherche Médicale, Unité 510, Faculté de Pharmacie Paris XI, F-92296 ChÂtenay-Malabry, France. Phone: 33 1 46 83 56 61. Fax: 33 1 46 83 58 44. E-mail: alain.servin{at}cep.u-psud.fr.

Editor: F. C. Fang

Infection and Immunity, June 2004, p. 3106-3112, Vol. 72, No. 6
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.6.3106-3112.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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