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Infection and Immunity, June 2004, p. 3429-3435, Vol. 72, No. 6
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.6.3429-3435.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Heterogeneity among Helicobacter pylori Strains in Expression of the Outer Membrane Protein BabA

Ewa E. Hennig,1 Ray Mernaugh,2 Jennifer Edl,2 Ping Cao,3 and Timothy L. Cover3,4,5*

Departments of Medicine,3 Microbiology and Immunology,4 Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232,2 Veterans Affairs Medical Center, Nashville, Tennessee 37212,5 Department of Gastroenterology, Medical Center for Postgraduate Education, Cancer Center, 02-781 Warsaw, Poland1

Received 24 October 2003/ Returned for modification 12 December 2003/ Accepted 16 February 2004

The BabA adhesin of Helicobacter pylori is an outer membrane protein that binds to the fucosylated Lewis b histo-blood group antigen on the surface of gastric epithelial cells. We screened a phage-displayed ScFv (single-chain fragment variable) recombinant antibody library for antibodies reactive with a recombinant BabA fragment and identified two such antibodies. Each antibody recognized an ~75-kDa protein present in wild-type H. pylori strain J99 but absent from an isogenic babA mutant strain. An immunoreactive BabA protein was detected by at least one of the antibodies in 18 (46%) of 39 different wild-type H. pylori strains and was detected more commonly in cagA-positive strains than in cagA-negative strains. Numerous amino acid polymorphisms were detected among BabA proteins expressed by different strains, with the greatest diversity occurring in the middle region of the proteins. Among the 18 strains that expressed a detectable BabA protein, there was considerable variation in the level of binding to Lewis b in vitro. Heterogeneity among H. pylori strains in expression of the BabA protein may be a factor that contributes to differing clinical outcomes among H. pylori-infected humans.

* Corresponding author. Mailing address: Division of Infectious Diseases, A3310 Medical Center North, Vanderbilt University School of Medicine, Nashville, TN 37232. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: timothy.L.cover{at}vanderbilt.edu.

Editor: J. B. Bliska

Infection and Immunity, June 2004, p. 3429-3435, Vol. 72, No. 6
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.6.3429-3435.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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