This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohnishi, H.
Right arrow Articles by Yoshida, S.-i.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohnishi, H.
Right arrow Articles by Yoshida, S.-i.

 Previous Article  |  Next Article 

Infection and Immunity, June 2004, p. 3592-3603, Vol. 72, No. 6
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.6.3592-3603.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Legionella dumoffii DjlA, a Member of the DnaJ Family, Is Required for Intracellular Growth

Hiroko Ohnishi,1,2 Yoshimitsu Mizunoe,1* Akemi Takade,1 Yoshitaka Tanaka,3 Hiroshi Miyamoto,4 Mine Harada,2 and Shin-ichi Yoshida1

Department of Bacteriology,1 Department of Medicine and Biosystemic Science, Internal Medicine,2 Faculty of Medical Sciences, and Division of Pharmaceutical Cell Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582,3 Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan4

Received 12 November 2003/ Returned for modification 5 January 2004/ Accepted 10 February 2004

Legionella dumoffii is one of the common causes of Legionnaires' disease and is capable of replicating in macrophages. To understand the mechanism of survival within macrophages, transposon mutagenesis was employed to isolate the genes necessary for intracellular growth. We identified four defective mutants after screening 790 transposon insertion mutants. Two transposon insertions were in genes homologous to icmB or dotC, within dot/icm loci, required for intracellular multiplication of L. pneumophila. The third was in a gene whose product is homologous to the 17-kDa antigen forming part of the VirB/VirD4 type IV secretion system of Bartonella henselae. The fourth was in the djlA (for "dnaj-like A") gene. DjlA is a member of the DnaJ/Hsp40 family. Transcomplementation of the djlA mutant restored the parental phenotype in J774 macrophages, A549 human alveolar epithelial cells, and the amoeba Acanthamoeba culbertsoni. Using confocal laser-scanning microscopy and transmission electron microscopy, we revealed that in contrast to the wild-type strain, L. dumoffii djlA mutant-containing phagosomes were unable to inhibit phagosome-lysosome fusion. Transmission electron microscopy also showed that in contrast to the virulent parental strain, the djlA mutant was not able to recruit host cell rough endoplasmic reticulum. Furthermore, the stationary-phase L. dumoffii djlA mutants were more susceptible to H2O2, high osmolarity, high temperature, and low pH than was their parental strain. These results indicate that DjlA is required for intracellular growth and organelle trafficking, as well as bacterial resistance to environmental stress. This is the first report demonstrating that a single DjlA-deficient mutant exhibits a distinct phenotype.

* Corresponding author. Mailing address: Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. Phone: 81-92-642-6128. Fax: 81-92-642-6133. E-mail: ymizunoe{at}bact.med.kyushu-u.ac.jp.

Editor: S. H. E. Kaufmann

Infection and Immunity, June 2004, p. 3592-3603, Vol. 72, No. 6
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.6.3592-3603.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moran-Barrio, J., Limansky, A. S., Viale, A. M. (2009). Secretion of GOB Metallo-{beta}-Lactamase in Escherichia coli Depends Strictly on the Cooperation between the Cytoplasmic DnaK Chaperone System and the Sec Machinery: Completion of Folding and Zn(II) Ion Acquisition Occur in the Bacterial Periplasm. Antimicrob. Agents Chemother. 53: 2908-2917 [Abstract] [Full Text]  
  • Aurass, P., Pless, B., Rydzewski, K., Holland, G., Bannert, N., Flieger, A. (2009). bdhA-patD Operon as a Virulence Determinant, Revealed by a Novel Large-Scale Approach for Identification of Legionella pneumophila Mutants Defective for Amoeba Infection. Appl. Environ. Microbiol. 75: 4506-4515 [Abstract] [Full Text]  
  • Lakhal, F., Bury-Mone, S., Nomane, Y., Le Goic, N., Paillard, C., Jacq, A. (2008). DjlA, a Membrane-Anchored DnaJ-Like Protein, Is Required for Cytotoxicity of Clam Pathogen Vibrio tapetis to Hemocytes. Appl. Environ. Microbiol. 74: 5750-5758 [Abstract] [Full Text]  
  • Vincent, C. D., Buscher, B. A., Friedman, J. R., Williams, L. A., Bardill, P., Vogel, J. P. (2006). Identification of Non-dot/icm Suppressors of the Legionella pneumophila {Delta}dotL Lethality Phenotype. J. Bacteriol. 188: 8231-8243 [Abstract] [Full Text]  
  • Cossins, A., Fraser, J., Hughes, M., Gracey, A. (2006). Post-genomic approaches to understanding the mechanisms of environmentally induced phenotypic plasticity. J. Exp. Biol. 209: 2328-2336 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»