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Infection and Immunity, July 2004, p. 3769-3776, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.3769-3776.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

Michael A. Jones,1* Kerrie L. Marston,1 Claire A. Woodall,2 Duncan J. Maskell,2 Dennis Linton,3 Andrey V. Karlyshev,4 Nick Dorrell,4 Brendan W. Wren,4 and Paul A. Barrow1

Institute for Animal Health, Compton, Newbury Berkshire RG20 7NN,1 Centre for Veterinary Sciences, University of Cambridge, Cambridge CB3 0ES,2 School of Biological Sciences, University of Manchester, Manchester M13 9PT,3 London School of Hygiene and Tropical Medicine, London WC1A 7HT, United Kingdom4

Received 14 October 2003/ Returned for modification 28 January 2004/ Accepted 31 March 2004

The genome sequence of the human pathogen Campylobacter jejuni NCTC11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that this strain is a poor colonizer. Experimental colonization and persistence studies were carried out with C. jejuni NCTC11168 by using 2-week-old Light Sussex chickens possessing an acquired natural gut flora. After inoculation, NCTC11168 initially colonized the intestine poorly. However, after 5 weeks we observed adaptation to high-level colonization, which was maintained after in vitro passage. The adapted strain exhibited greatly increased motility. A second strain, C. jejuni 11168H, which had been selected under in vitro conditions for increased motility (A. V. Karlyshev, D. Linton, N. A. Gregson, and B. W. Wren, Microbiology 148:473-480, 2002), also showed high-level intestinal colonization. The levels of colonization were equivalent to those of six other strains, assessed under the same conditions. There were four mutations in C. jejuni 11168H that reduced colonization; maf5, flaA (motility and flagellation), and kpsM (capsule deficiency) eliminated colonization, whereas pglH (general glycosylation system deficient) reduced but did not eliminate colonization. This study showed that there was colonization of the avian intestinal tract by a Campylobacter strain having a known genome sequence, and it provides a model for colonization and persistence studies with specific mutations.

* Corresponding author. Mailing address: Institute for Animal Health, Compton, Newbury Berkshire RG20 7NN, United Kingdom. Phone: (1635) 578411. Fax: (1635) 577243. E-mail: majones{at}bbsrc.ac.uk.

Editor: V. J. DiRita

Infection and Immunity, July 2004, p. 3769-3776, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.3769-3776.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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