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Infection and Immunity, July 2004, p. 3876-3882, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.3876-3882.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Functional Analysis of the Streptococcus gordonii DL1 Sialic Acid-Binding Adhesin and Its Essential Role in Bacterial Binding to Platelets

Yukihiro Takahashi,1* Ayako Yajima,1 John O. Cisar,2 and Kiyoshi Konishi1

Department of Microbiology, The Nippon Dental University School of Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan,1 Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, Bethesda, Maryland 20892-43522

Received 7 January 2004/ Returned for modification 18 February 2004/ Accepted 31 March 2004

Bacterial recognition of host sialic acid-containing receptors plays an important role in microbial colonization of the human oral cavity. The sialic acid-binding adhesin of Streptococcus gordonii DL1 was previously associated with the hsa gene encoding a 203-kDa protein. The predicted protein sequence consists of an N-terminal nonrepetitive region (NR1), including a signal sequence, a relatively short serine-rich region (SR1), a second nonrepetitive region (NR2), a long serine-rich region (SR2) containing 113 dodecapeptide repeats, and a C-terminal cell wall anchoring domain. In the present study, the contributions of SR1, NR2, and SR2 to Hsa-mediated adhesion were assessed by genetic complementation. Adhesion of an hsa chromosomal deletion mutant to sialic acid-containing receptors was restored by plasmids containing hsa constructs encoding Hsa that lacked either the N- or C-terminal portion of SR2. In contrast, hsa constructs that lacked the coding sequences for SR1, NR2, or the entire SR2 region failed to restore adhesion. Surface expression of recombinant Hsa was not affected by removal of SR1, NR2, or a portion of SR2 but was greatly reduced by complete removal of SR2. Wheat germ agglutinin, a probe for Hsa-specific glycosylation, reacted with recombinant Hsa lacking SR1, NR2, or SR2 but not with recombinant Hsa lacking both SR1 and SR2. Significantly, the aggregation of human platelets by S. gordonii DL1, an interaction implicated in the pathogenesis of infective endocarditis, required the expression of hsa. Moreover, neuraminidase treatment of the platelets eliminated this interaction, further supporting the hypothesis that Hsa plays an essential role in the bacterium-platelet interaction.

* Corresponding author. Mailing address: Department of Microbiology, The Nippon Dental University School of Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan. Phone: 81-3-3261-8763. Fax: 81-3-3264-8399. E-mail: biseibut{at}tky.ndu.ac.jp.

Editor: J. N. Weiser

Infection and Immunity, July 2004, p. 3876-3882, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.3876-3882.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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