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Infection and Immunity, July 2004, p. 3883-3889, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.3883-3889.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Anaplasma phagocytophilum Has a Functional msp2 Gene That Is Distinct from p44

Quan Lin,1 Yasuko Rikihisa,1* Suleyman Felek,1 Xueqi Wang,1 Robert F. Massung,2 and Zerai Woldehiwet3

Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio,1 Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,2 Department of Veterinary Pathology, University of Liverpool, Leahurst, Neston, Wirral, United Kingdom3

Received 21 January 2004/ Returned for modification 19 February 2004/ Accepted 14 March 2004

The msp2 and p44 genes encode polymorphic major outer membrane proteins that are considered unique to the intraerythrocytic agent of Anaplasma marginale and the intragranulocytic agent of Anaplasma phagocytophilum, respectively. In the present study, however, we found an msp2 gene in A. phagocytophilum that was remarkably conserved among A. phagocytophilum strains from human granulocytic anaplasmosis (HGA) patients, ticks, and a horse from various regions in the United States, but the gene was different in a sheep isolate from the United Kingdom. The msp2 gene in the A. phagocytophilum strain HZ genome was a single-copy gene and was located downstream of two Ehrlichia chaffeensis omp-1 homologs and a decarboxylase gene (ubiD). The msp2 gene was expressed by A. phagocytophilum in the blood from HGA patients NY36 and NY37 and by A. phagocytophilum isolates from these patients cultured in HL-60 cells at 37°C. The msp2 gene was also expressed in a DBA/2 mouse infected by attaching ticks infected with strain NTN-1 and in a horse experimentally infected by attaching strain HZ-infected ticks. However, the transcript of the msp2 gene was undetectable in A. phagocytophilum strain HZ in SCID mice and Ixodes scapularis ticks infected with strain NTN-1. These results indicate that msp2 is functional in various strains of A. phagocytophilum, and relative expression ratios of msp2 to p44 vary in different infected hosts. These findings may be important in understanding roles that Msp2 proteins play in granulocytic ehrlichia infection and evolution of the polymorphic major outer membrane protein gene families in Anaplasma species.

* Corresponding author. Mailing address: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd., Columbus, OH 43210-1093. Phone: (614) 292-5661. Fax: (614) 292-6473. E-mail: rikihisa.1{at}osu.edu.

Editor: J. T. Barbieri

Infection and Immunity, July 2004, p. 3883-3889, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.3883-3889.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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