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Infection and Immunity, July 2004, p. 4004-4009, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4004-4009.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Yersinia enterocolitica Type III Secretion Depends on the Proton Motive Force but Not on the Flagellar Motor Components MotA and MotB

Gottfried Wilharm,* Verena Lehmann,{dagger} Kristina Krauss, Beatrix Lehnert, Susanna Richter, Klaus Ruckdeschel, Jürgen Heesemann, and Konrad Trülzsch

Max von Pettenkofer-Institut, D-80336 Munich, Germany

Received 2 December 2003/ Returned for modification 6 January 2004/ Accepted 15 March 2004

The flagellum is believed to be the common ancestor of all type III secretion systems (TTSSs). In Yersinia enterocolitica, expression of the flagellar TTSS and the Ysc (Yop secretion) TTSS are inversely regulated. We therefore hypothesized that the Ysc TTSS may adopt flagellar motor components in order to use the pathogenicity-related translocon in a drill-like manner. As a prerequisite for this hypothesis, we first tested a requirement for the proton motive force by both systems using the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP). Motility as well as type III-dependent secretion of Yop proteins was inhibited by CCCP. We deleted motAB, which resulted in an immotile phenotype. This mutant, however, secreted amounts of Yops to the supernatant comparable to those of the wild type. Translocation of Yops into host cells was also not affected by the motAB deletion. Virulence of the mutant was comparable to that of the wild type in the mouse oral infection model. Thus, the hypothesis that the Ysc TTSS might adopt flagellar motor components was not confirmed. The finding that, in addition to consumption of ATP, Ysc TTSS requires the proton motive force is discussed.

* Corresponding author. Mailing address: Bakteriologie, Max von Pettenkofer-Institut, Pettenkoferstrasse 9a, D-80336 Munich, Germany. Phone: 49 (89) 5160 5279. Fax: 49 (89) 5160 5223. E-mail: maier{at}m3401.mpk.med.uni-muenchen.de.

Editor: J. B. Bliska

{dagger} Present address: Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin, Germany.

Infection and Immunity, July 2004, p. 4004-4009, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4004-4009.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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