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Infection and Immunity, July 2004, p. 4109-4113, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4109-4113.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Protection Afforded by Heat Shock Protein 60 from Francisella tularensis Is Due to Copurified Lipopolysaccharide

M. G. Hartley,^1* M. Green,¹ G. Choules,¹ D. Rogers,¹ D. G. C. Rees,¹ S. Newstead,¹ A. Sjostedt,² and R. W. Titball^1,3

Defence Science and Technology Laboratory, Porton Down, Salisbury, Wilts SP4 OJQ,¹ Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom,³ Department of Clinical Bacteriology, Umea University, S-901 85 Umea, Sweden²

Received 10 October 2003/ Returned for modification 12 January 2004/ Accepted 31 March 2004

Heat shock proteins (Hsps) have attracted significant attention as protective antigens against a range of diseases caused by bacterial pathogens. However, more recently there have been suggestions that the protective response is due to the presence of peptide components other than Hsps. We have shown that mice that had been immunized with purified heat shock protein 60 (Hsp60) isolated from Francisella tularensis were protected against a subsequent challenge with some strains of the bacterium. However, this protection appeared to be due to trace amounts of lipopolysaccharide, which were too low to be detected by using the Limulus amoebocyte lysate assay. This finding raises the possibility that the protection afforded by other bacterial Hsp60 proteins may be due to trace quantities of polysaccharide antigens carried by and acting in conjunction with the Hsps.

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* Corresponding author. Mailing address: Bldg. 245, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wilts SP4 0NS, United Kingdom. Phone: 44 1980 613462. Fax: 44 1980 613284. E-mail: mghartley{at}dstl.gov.uk.

Editor: V. J. DiRita

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Infection and Immunity, July 2004, p. 4109-4113, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4109-4113.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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