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Infection and Immunity, July 2004, p. 4114-4126, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4114-4126.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Description of the Transcriptomes of Immune Response-Activated Hemocytes from the Mosquito Vectors Aedes aegypti and Armigeres subalbatus

Lyric C. Bartholomay,1,{dagger} Wen-Long Cho,2,{dagger} Thomas A. Rocheleau,1 Jon P. Boyle,1 Eric T. Beck,1 Jeremy F. Fuchs,1 Paul Liss,1 Michael Rusch,1 Katherine M. Butler,1 Roy Chen-Chih Wu,2 Shih-Pei Lin,2 Hang-Yen Kuo,2 I-Yu Tsao,2 Chiung-Yin Huang,2 Tze-Tze Liu,3 Kwang-Jen Hsiao,3 Shih-Feng Tsai,4 Ueng-Cheng Yang,5 Anthony J. Nappi,1 Nicole T. Perna,1 Chen-Cheng Chen,2* and Bruce M. Christensen1*

Department of Animal Health and Biomedical Sciences, University of Wisconsin—Madison, Madison, Wisconsin 53706,1 Department of Parasitology,2 Genome Research Center and Institute of Genetics,3 Institute of Bioinformatics, National Yang-Ming University, Shih-Pai, Taipei 112,5 National Health Research Institutes, Nankang, Taipei 115, Taiwan4

Received 27 January 2004/ Returned for modification 5 March 2004/ Accepted 21 March 2004

Mosquito-borne diseases, including dengue, malaria, and lymphatic filariasis, exact a devastating toll on global health and economics, killing or debilitating millions every year (54). Mosquito innate immune responses are at the forefront of concerted research efforts aimed at defining potential target genes that could be manipulated to engineer pathogen resistance in vector populations. We aimed to describe the pivotal role that circulating blood cells (called hemocytes) play in immunity by generating a total of 11,952 Aedes aegypti and 12,790 Armigeres subalbatus expressed sequence tag (EST) sequences from immune response-activated hemocyte libraries. These ESTs collapsed into 2,686 and 2,107 EST clusters, respectively. The clusters were used to adapt the web-based interface for annotating bacterial genomes called A Systematic Annotation Package for Community Analysis of Genomes (ASAP) for analysis of ESTs. Each cluster was categorically characterized and annotated in ASAP based on sequence similarity to five sequence databases. The sequence data and annotations can be viewed in ASAP at https://asap.ahabs.wisc.edu/annotation/php/ASAP1.htm. The data presented here represent the results of the first high-throughput in vivo analysis of the transcriptome of immunocytes from an invertebrate. Among the sequences are those for numerous immunity-related genes, many of which parallel those employed in vertebrate innate immunity, that have never been described for these mosquitoes.

* Corresponding author. Mailing address for Bruce M. Christensen: Department of Animal Health and Biomedical Sciences, University of Wisconsin—Madison, 1656 Linden Dr., Madison, WI 53706. Phone: (608) 262-3850. Fax: (608) 262-7420. E-mail: christensen{at}svm.vetmed.wisc.edu. Mailing address for Chen-Cheng Chen: Department of Parasitology, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan. Phone: 886-2-2821-3892. Fax: 886-2-2821-4670. E-mail: mosquito{at}ym.edu.tw.

Editor: W. A. Petri, Jr.

{dagger} L.C.B. and W.-L.C. contributed equally to the present work.

Infection and Immunity, July 2004, p. 4114-4126, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4114-4126.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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