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Infection and Immunity, July 2004, p. 4302-4308, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4302-4308.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Function of the Fibronectin-Binding Serum Opacity Factor of Streptococcus pyogenes in Adherence to Epithelial Cells

Sonja Oehmcke,1 Andreas Podbielski,1 and Bernd Kreikemeyer1,2*

Department of Medical Microbiology and Hospital Hygiene, University Clinic Rostock, Rostock,1 Department of Microbial Pathogenesis and Vaccine Research, Technical University/GBF-National Research Center for Biotechnology, Braunschweig, Germany2

Received 20 February 2004/ Accepted 23 March 2004

The serum opacity factor (SOF) of Streptococcus pyogenes is a serotyping tool and pathogenesis factor. Using SOF-coated latex beads in cell adherence assays and antiserum directed against SOF in S. pyogenes-HEp-2 cell adherence inhibition experiments, we demonstrate SOF involvement in the fibronectin-mediated adherence of S. pyogenes to epithelial cells. SOF exclusively targets the 30-kDa N-terminal region of fibronectin. The interaction revealed association and dissociation constants 1 order of magnitude lower than those of other S. pyogenes fibronectin-binding proteins.

* Corresponding author. Mailing address: University Hospital Rostock, Department of Medical Microbiology and Hospital Hygiene, Schillingallee 70, 18055 Rostock, Germany. Phone: 49-381-494-5912. Fax: 49-381-494-5902. E-mail: Bernd.Kreikemeyer{at}med.uni-rostock.de.

Editor: V. J. DiRita

Infection and Immunity, July 2004, p. 4302-4308, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4302-4308.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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