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Infection and Immunity, July 2004, p. 4309-4313, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4309-4313.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Immunization with Native or Recombinant Streptococcus pneumoniae Neuraminidase Affords Protection in the Chinchilla Otitis Media Model

J. P. Long, H. H. Tong, and T. F. DeMaria*

Division of Otologic Research, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio

Received 14 October 2003/ Returned for modification 26 November 2003/ Accepted 17 March 2004

Streptococcus pneumoniae neuraminidase has been implicated as a virulence factor in the pathogenesis of pneumococcal otitis media. In this study, native neuraminidase was partially purified from cultures of S. pneumoniae by serial chromatography with DEAE-Sepharose and Sephacryl S-200. Recombinant neuraminidase, a 3,038-bp fragment of the neuraminidase A (nanA) gene, was cloned into the pET-28b vector and then expressed at high levels in Escherichia coli. Chinchillas were immunized subcutaneously with either the gel-purified native or recombinant neuraminidase, and all responded with elevated titers of antineuraminidase antibody in serum. Immunization with neuraminidase resulted in a significant reduction in nasopharyngeal colonization as well as in the incidence of otitis media with effusion. These data demonstrate for the first time that neuraminidase affords protection against S. pneumoniae nasopharyngeal colonization and experimental otitis media.

* Corresponding author. Mailing address: Division of Otologic Research, College of Medicine and Public Health, The Ohio State University, Room 4331, Cramblett Hall, 456 W. 10th Ave., Columbus, OH 43210. Phone: (614) 293-8103. Fax: (614) 293-5506. E-mail: demaria.2{at}osu.edu.

Editor: J. N. Weiser

Infection and Immunity, July 2004, p. 4309-4313, Vol. 72, No. 7
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.7.4309-4313.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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