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Infection and Immunity, August 2004, p. 4612-4618, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4612-4618.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Gamma Interferon Production by Bovine T Cells following Stimulation with Mycobacterial Mycolylarabinogalactan Peptidoglycan

B. Vesosky,^* O. C. Turner, J. Turner, and I. M. Orme

Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523

Received 10 October 2003/ Returned for modification 13 December 2003/ Accepted 18 April 2004

A large percentage of lymphocytes in the blood of cattle express the T-cell receptor, but specific functions for these cells have not yet been clearly defined. There is evidence, however, that human, murine, and bovine T cells have a role in the immune response to mycobacteria. This study investigated the ability of bovine T cells to expand and produce gamma interferon (IFN-) in response to stimulation with mycobacterial products. Bovine T cells, isolated from the peripheral blood of healthy cattle, expanded following in vitro stimulation with live mycobacteria, mycobacterial crude cell wall extract, and Mycobacterium bovis culture filtrate proteins. In addition, purified T cells, cocultured with purified monocytes and interleukin-2, consistently produced significant amounts of IFN- in response to mycobacterial cell wall. The IFN--inducing component of the cell wall was further identified as a proteolytically resistant, non-sodium dodecyl sulfate-soluble component of the mycolylarabinogalactan peptidoglycan.

Editor: J. F. Urban, Jr.

Present address: Novartis Pharmaceuticals Corporation, East Hanover, NJ 07936.

Present address: Department of Internal Medicine, Center for Microbial Interface Biology, The Ohio State University, Columbus, OH 43210.

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