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Infection and Immunity, August 2004, p. 4699-4706, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4699-4706.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Contribution of the Alanine-Rich Region of Streptococcus mutans P1 to Antigenicity, Surface Expression, and Interaction with the Proline-Rich Repeat Domain

Trevor B. Seifert, Arnold S. Bleiweis, and L. Jeannine Brady*

Department of Oral Biology, University of Florida, Gainesville, Florida 32610

Received 4 February 2004/ Returned for modification 16 March 2004/ Accepted 22 April 2004

Streptococcus mutans is considered to be the major etiologic agent of human dental caries. Attachment of S. mutans to the tooth surface is required for the development of caries and is mediated, in part, by the 185-kDa surface protein variously known as antigen I/II, PAc, and P1. Such proteins are expressed by nearly all species of oral streptococci. Characteristics of P1 include an alanine-rich repeat region and a centrally located proline-rich repeat region. The proline-rich region of P1 has been shown to be important for the translational stability and translocation of P1 through the bacterial membrane. We show here that (i) several anti-P1 monoclonal antibodies require the simultaneous presence of the alanine-rich and proline-rich regions for binding, (ii) the proline-rich region of P1 interacts with the alanine-rich region, (iii) like the proline-rich region, the alanine-rich region is required for the stability and translocation of P1, (iv) both the proline-rich and alanine-rich regions are required for secretion of P1 in Escherichia coli, and (v) in E. coli, P1 is secreted in the absence of SecB.

* Corresponding author. Mailing address: Department of Oral Biology, University of Florida, P.O. Box 100424 D4-20, Gainesville, FL 32610-0424. Phone: (352) 846-0785. Fax: (352) 846-0786. E-mail: jbrady{at}dental.ufl.edu.

Editor: J. D. Clements

Infection and Immunity, August 2004, p. 4699-4706, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4699-4706.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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