This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Keller, C. C.
Right arrow Articles by Perkins, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Keller, C. C.
Right arrow Articles by Perkins, D. J.

 Previous Article  |  Next Article 

Infection and Immunity, August 2004, p. 4868-4873, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4868-4873.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Elevated Nitric Oxide Production in Children with Malarial Anemia: Hemozoin-Induced Nitric Oxide Synthase Type 2 Transcripts and Nitric Oxide in Blood Mononuclear Cells

Christopher C. Keller,1 Peter G. Kremsner,2,3 James B. Hittner,4 Mary A. Misukonis,5 J. Brice Weinberg,5 and Douglas J. Perkins1*

Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania,1 Medical Research Unit, Albert Schweitzer Hospital, Lambaréné, Gabon,2 Department of Parasitology, Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany,3 Department of Psychology, College of Charleston, Charleston, South Carolina,4 Department of Medicine, Durham VA and Duke University Medical Centers, Durham, North Carolina5

Received 16 February 2004/ Returned for modification 28 March 2004/ Accepted 9 April 2004

Experiments outlined here investigate the role of nitric oxide (NO) in the pathogenesis of Plasmodium falciparum-induced malarial anemia (MA). The results show that ex vivo and in vitro NO synthase (NOS) activity in peripheral blood mononuclear cells (PBMCs) is significantly elevated in children with MA and inversely associated with hemoglobin levels. Additional experiments using PBMCs from non-malaria-exposed donors demonstrate that physiologic amounts of P. falciparum-derived hemozoin augment NOS type 2 (NOS2) transcripts and NO production. Results of these experiments illustrate that elevated NO production in children with MA is associated with decreased hemoglobin concentrations and that hemozoin can induce NOS2-derived NO formation in cultured blood mononuclear cells.

* Corresponding author. Mailing address: 603 Parran Hall, 130 DeSoto St., Pittsburgh, PA 15261. Phone: (412) 624-5894. Fax: (412) 624-4953. E-mail: djp{at}pitt.edu.

Editor: W. A. Petri, Jr.

Infection and Immunity, August 2004, p. 4868-4873, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4868-4873.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Akman-Anderson, L., Olivier, M., Luckhart, S. (2007). Induction of Nitric Oxide Synthase and Activation of Signaling Proteins in Anopheles Mosquitoes by the Malaria Pigment, Hemozoin. Infect. Immun. 75: 4012-4019 [Abstract] [Full Text]  
  • Awandare, G. A., Ouma, Y., Ouma, C., Were, T., Otieno, R., Keller, C. C., Davenport, G. C., Hittner, J. B., Vulule, J., Ferrell, R., Ong'echa, J. M., Perkins, D. J. (2007). Role of Monocyte-Acquired Hemozoin in Suppression of Macrophage Migration Inhibitory Factor in Children with Severe Malarial Anemia. Infect. Immun. 75: 201-210 [Abstract] [Full Text]  
  • Casals-Pascual, C., Kai, O., Cheung, J. O. P., Williams, S., Lowe, B., Nyanoti, M., Williams, T. N., Maitland, K., Molyneux, M., Newton, C. R. J. C., Peshu, N., Watt, S. M., Roberts, D. J. (2006). Suppression of erythropoiesis in malarial anemia is associated with hemozoin in vitro and in vivo. Blood 108: 2569-2577 [Abstract] [Full Text]  
  • Keller, C. C., Yamo, O., Ouma, C., Ong'echa, J. M., Ounah, D., Hittner, J. B., Vulule, J. M., Perkins, D. J. (2006). Acquisition of Hemozoin by Monocytes Down-Regulates Interleukin-12 p40 (IL-12p40) Transcripts and Circulating IL-12p70 through an IL-10-Dependent Mechanism: In Vivo and In Vitro Findings in Severe Malarial Anemia. Infect. Immun. 74: 5249-5260 [Abstract] [Full Text]  
  • ZAMORA, F., RAMIREZ, O., VERGARA, J., AREVALO-HERRERA, M., HERRERA, S. (2005). HEMOGLOBIN LEVELS RELATED TO DAYS OF ILLNESS, RACE, AND PLASMODIUM SPECIES IN COLOMBIAN PATIENTS WITH UNCOMPLICATED MALARIA. Am J Trop Med Hyg 73: 50-54 [Abstract] [Full Text]  
  • Ochiel, D. O., Awandare, G. A., Keller, C. C., Hittner, J. B., Kremsner, P. G., Weinberg, J. B., Perkins, D. J. (2005). Differential Regulation of {beta}-Chemokines in Children with Plasmodium falciparum Malaria. Infect. Immun. 73: 4190-4197 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»