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Infection and Immunity, August 2004, p. 4911-4917, Vol. 72, No. 8
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.8.4911-4917.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Intact Purine Biosynthesis Pathways Are Required for Wild-Type Virulence of Brucella abortus 2308 in the BALB/c Mouse Model

Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27858-4354

Received 28 October 2003/ Returned for modification 12 January 2004/ Accepted 12 May 2004

Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn5 insertions in pheA, trpB, and dagA display little or no attenuation in cultured murine macrophages or mice. These experimental findings confirm the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages. In contrast to previous reports, however, these results indicate that exogenous tryptophan and phenylalanine are available for use by the brucellae in the phagosomal compartment.

Editor: V. J. DiRita

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