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Infection and Immunity, September 2004, p. 5080-5088, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5080-5088.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Evolutionary Relationships of Enteroinvasive Escherichia coli and Shigella spp.

Ruiting Lan,1* M. Chehani Alles,2 Kathy Donohoe,2 Marina B. Martinez,3 and Peter R. Reeves2*

School of Biotechnology and Biomolecular Sciences, University of New South Wales,1 School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia,2 Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo-SP, Sao Paulo, Brazil3

Received 9 December 2003/ Returned for modification 10 May 2004/ Accepted 7 June 2004

Enteroinvasive Escherichia coli (EIEC), a distinctive pathogenic form of E. coli causing dysentery, is similar in many properties to bacteria placed in the four species of Shigella. Shigella has been separated as a genus but in fact comprises several clones of E. coli. The evolutionary relationships of 32 EIEC strains of 12 serotypes have been determined by sequencing of four housekeeping genes and two plasmid genes which were used previously to determine the relationships of Shigella strains. The EIEC strains were grouped in four clusters with one outlier strain, indicating independent derivation of EIEC several times. Three of the four clusters contain more than one O antigen type. One EIEC strain (an O112ac:H– strain) was found in Shigella cluster 3 but is not identical to the Shigella cluster 3 D2 and B15 strains with the same O antigen. Two forms of the virulence plasmid pINV have been identified in Shigella strains by using the sequences of ipgD and mxiA genes, and all but two of our EIEC strains have pINV A. The EIEC strains were grouped in two subclusters with a very low level of variation, generally not intermingled with Shigella pINV A strains. The EIEC clusters based on housekeeping genes were reflected in the plasmid gene sequences, with some exceptions. Two strains were found in the pINV B form by using the ipgD sequence, with one strain having an mxiA sequence similar to the divergent sequence of D1. Clearly, EIEC and Shigella spp. form a pathovar of E. coli.

* Corresponding author. Mailing address: School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia. Phone: 61-2 9385 2095. Fax: 61-2 9385 1591. E-mail: r.lan{at}unsw.edu.au or reeves{at}angis.usyd.edu.au.

Editor: V. J. DiRita

Infection and Immunity, September 2004, p. 5080-5088, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5080-5088.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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