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Infection and Immunity, September 2004, p. 5452-5459, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5452-5459.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Transcriptome of Enterohemorrhagic Escherichia coli O157 Adhering to Eukaryotic Plasma Membranes

Sivan Dahan,1 Stuart Knutton,2 Robert K. Shaw,2 Valerie F. Crepin,1 Gordon Dougan,1 and Gad Frankel1*

Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London,1 Institute of Child Health, University of Birmingham, Birmingham, United Kingdom2

Received 17 December 2003/ Returned for modification 31 January 2004/ Accepted 17 May 2004

Using a DNA microarray, we determined changes in enterohemorrhagic Escherichia coli O157:H7 gene expression during binding to plasma membranes. Analysis of the complete transcriptomes of the bound bacteria revealed increased levels of stress-associated mRNAs and decreased levels of mRNA encoding proteins involved in translation and type III secretion.

* Corresponding author. Mailing address: Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, United Kingdom. Phone: 44 (0) 20 7594 5253. Fax: 44 (0) 20 7594 3069. E-mail: g.frankel{at}imperial.ac.uk.

Editor: J. B. Bliska

Infection and Immunity, September 2004, p. 5452-5459, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5452-5459.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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