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Infection and Immunity, September 2004, p. 5493-5497, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5493-5497.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of a Novel Chloramphenicol Resistance Expression Plasmid Used for Genetic Complementation of a fliG Deletion Mutant in Treponema denticola

Linda L. Slivienski-Gebhardt, Jacques Izard,{dagger} William A. Samsonoff, and Ronald J. Limberger*

David Axelrod Institute for Public Health, Wadsworth Center, New York State, Department of Health, Albany, New York

Received 2 April 2004/ Returned for modification 4 May 2004/ Accepted 9 June 2004

A new expression plasmid containing the fla operon promoter and a staphylococcal chloramphenicol resistance gene, was constructed to help assess the role of fliG in Treponema denticola motility. Deletion of fliG resulted in a nonmotile mutant with a markedly decreased number of flagellar filaments. Wild-type fliG genes from T. denticola and from Treponema pallidum were cloned into this expression plasmid. In both cases, the gene restored the ability of the mutant to gyrate its cell ends and enabled colony spreading in agarose. This shuttle plasmid enables high-level expression of genes in T. denticola and possesses an efficient selectable marker that provides a new tool for treponemal genetics.

* Corresponding author. Mailing address: David Axelrod Institute for Public Health, Wadsworth Center, New York State, Department of Health, P.O. Box 22002, Albany, NY 12201-2002. Phone: (518) 474-4177. Fax: (518) 486-7971. E-mail: Ron.Limberger{at}wadsworth.org.

Editor: J. T. Barbieri

{dagger} Present address: The Forsyth Institute, Boston, MA 02115.

Infection and Immunity, September 2004, p. 5493-5497, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5493-5497.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cameron, C. E., Kuroiwa, J. M. Y., Yamada, M., Francescutti, T., Chi, B., Kuramitsu, H. K. (2008). Heterologous Expression of the Treponema pallidum Laminin-Binding Adhesin Tp0751 in the Culturable Spirochete Treponema phagedenis. J. Bacteriol. 190: 2565-2571 [Abstract] [Full Text]  


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