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Infection and Immunity, September 2004, p. 5506-5510, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5506-5510.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Growth Phase Regulation of flaA Expression in Helicobacter pylori Is luxS Dependent

John T. Loh,1 Mark H. Forsyth,2 and Timothy L. Cover1,3,4*

Departments of Medicine,1 Microbiology and Immunology, Vanderbilt University Medical Center, and,3 Veterans Affair Medical Center, Nashville, Tennessee,4 Department of Biology, The College of William and Mary, Williamsburg, Virginia2

Received 15 December 2003/ Returned for modification 15 March 2004/ Accepted 20 May 2004

LuxS plays a role in the synthesis of an extracellular signaling molecule, autoinducer 2 (AI-2). To analyze a possible role of AI-2 in regulating Helicobacter pylori gene expression, we constructed a panel of transcriptional reporter strains. We show that the expression of H. pylori flaA is growth phase dependent and that flaA transcription increases in association with increased culture density. Mutating the luxS gene eliminates growth-phase-dependent control of flaA, and this growth phase dependence is restored when the luxS mutant strain is complemented with the wild-type luxS gene.

* Corresponding author. Mailing address: Department of Infectious Diseases, A3310 Medical Center N., Vanderbilt University School of Medicine, Nashville, TN 37232. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: timothy.L.cover{at}vanderbilt.edu.

Editor: V. J. DiRita

Infection and Immunity, September 2004, p. 5506-5510, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5506-5510.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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