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Infection and Immunity, September 2004, p. 5522-5525, Vol. 72, No. 9
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.9.5522-5525.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Salmonella enterica Serovar Typhimurium Divalent Cation Transport Systems MntH and SitABCD Are Essential for Virulence in an Nramp1^G169 Murine Typhoid Model

Michelle L. Zaharik,^1,2, Vivian Li Cullen,³ Angela M. Fung,³ Stephen J. Libby,⁴ Sonya L. Kujat Choy,² Bryan Coburn,² David G. Kehres,⁵ Michael E. Maguire,⁵ Ferric C. Fang,^3,4 and B. Brett Finlay^1,2,6*

Department of Biochemistry and Molecular Biology,¹ Biotechnology Laboratory,² Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada,⁶ Departments of Laboratory Medicine,³ Microbiology, University of Washington, Seattle, Washington,⁴ Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio⁵

Received 29 March 2004/ Returned for modification 28 April 2004/ Accepted 14 June 2004

Nramp1 is a transporter that pumps divalent cations from the vacuoles of phagocytic cells and is associated with the innate resistance of mice to diverse intracellular pathogens. We demonstrate that sitA and mntH, genes encoding high-affinity metal ion uptake systems in Salmonella enterica serovar Typhimurium, are upregulated when Salmonella is internalized by Nramp1-expressing macrophages and play an essential role in systemic infection of congenic Nramp1-expressing mice.

Editor: A. D. O'Brien

Present address: Chlamydia Research Laboratory, UBC CDC, Vancouver, BC V5Z 4R4, Canada.

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