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Infection and Immunity, January 2005, p. 638-643, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.638-643.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of an ExoS Type III Translocation-Resistant Cell Line

Elizabeth A. Rucks and Joan C. Olson*

Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, West Virginia University, Morgantown, West Virginia

Received 11 July 2004/ Returned for modification 4 August 2004/ Accepted 9 September 2004

Pseudomonas aeruginosa ExoS is a type III-secreted type III-secreted, bifunctional protein that causes diverse effects on eukaryotic cell function. The coculture of P. aeruginosa strains expressing ExoS with HL-60 myeloid cells revealed the cell line to be resistant to the toxic effects of ExoS. Differentiation of HL-60 cells with phorbol 12-myristate 13-acetate (TPA) rendered the cell line sensitive to ExoS. To understand the cellular basis for the alteration in sensitivity, undifferentiated and TPA-differentiated HL-60 cells were compared for differences in bacterial adherence, type III secretion induction, and ExoS translocation. These comparisons found that ExoS was translocated more efficiently in TPA-differentiated HL-60 cells than in undifferentiated cells. The studies support the ability of eukaryotic cells to influence P. aeruginosa TTS at the level of membrane translocation.

* Corresponding author. Mailing address: Department of Microbiology, Immunology, and Cell Biology, Robert C. Byrd Health Sciences Center, P.O. Box 9177, West Virginia University, Morgantown, WV 26506-9177. Phone: (304) 293-5843. Fax: (304) 293-7823. E-mail: jolson{at}hsc.wvu.edu.

Editor: D. L. Burns

Infection and Immunity, January 2005, p. 638-643, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.638-643.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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Copyright © 2005 by the American Society for Microbiology. All rights reserved.