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 Previous Article

Infection and Immunity, January 2005, p. 666-670, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.666-670.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Reduced Local Growth and Spread but Preserved Pathogenicity of a {Delta}purC Mycobacterium tuberculosis Auxotrophic Mutant in Gamma Interferon Receptor-Deficient Mice after Aerosol Infection

Najmeeyah Brown,1 Muazzam Jacobs,1 Shreemanta K. Parida,2 Tania Botha,3 Aldina Santos,3 Lizette Fick,1 Brigitte Gicquel,4 Mary Jackson,4 Valerie Quesniaux,5 and Bernhard Ryffel5*

Department of Immunology, Department of Health Sciences, University of Cape Town,1 Cape Technikon, Cape Town, South Africa,3 Armauer Hansen Research Institute, Addis Ababa, Ethiopia,2 Pasteur Institute, Paris,4 Centre National de la Recherche Scientifique, Orléans, France5

Received 10 May 2004/ Returned for modification 14 June 2004/ Accepted 2 August 2004

We compared the growth levels and pathogenicities of the Mycobacterium tuberculosis MT103 clinical strain with those of the {Delta}purC mutant strain MYC1551, which is auxotrophic for purine in wild-type and gamma interferon receptor (IFN-{gamma}R)-deficient mice. The {Delta}purC strain MYC1551 grew initially in both wild-type and IFN-{gamma}R-deficient mice upon aerosol infection, but it grew much less than strain MT103 did. Despite the comparable bacterial burdens of the mice, IFN-{gamma}R-deficient mice succumbed to infection with {Delta}purC strain MYC1551 from necrotic pneumonia within 6 weeks of those infected with MT103. In conclusion, the {Delta}purC mutant MYC1551 displays reduced growth but retains pathogenicity. Therefore, the use of mutant strains of M. tuberculosis as live vaccines may not be recommended.

* Corresponding author. Mailing address: CNRS, 3B rue de la Ferollerie, F-45071 Orléans, France. Phone: 0033 238 25 78 60. Fax: 0033 238 25 79 79. E-mail: bryffel{at}cnrs-orleans.fr.

Editor: F. C. Fang

Infection and Immunity, January 2005, p. 666-670, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.666-670.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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