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Infection and Immunity, January 2005, p. 79-87, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.79-87.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Ehrlichia chaffeensis Expresses Macrophage- and Tick Cell-Specific 28-Kilodalton Outer Membrane Proteins{dagger}

Vijayakrishna Singu, Haijie Liu, Chuanmin Cheng, and Roman R. Ganta*

Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas

Received 26 July 2004/ Returned for modification 26 August 2004/ Accepted 9 September 2004

Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, causes human monocyte/macrophage-tropic ehrlichiosis. In this study, proteomic approaches were used to demonstrate host cell-specific antigenic expression by E. chaffeensis. The differentially expressed antigens include those from the 28-kDa outer membrane protein (p28-Omp) multigene locus. The proteins expressed in infected macrophages are the products of p28-Omp19 and p28-Omp20 genes, whereas in tick cells, the protein expressed is the p28-Omp14 gene product. The differentially expressed proteins are posttranslationally modified by phosphorylation and glycosylation to generate multiple expressed forms. Host cell-specific protein expression is not influenced by growth temperatures and is reversible. Host cell-specific protein expression coupled with posttranslational modifications may be a hallmark for the pathogen's adaptation to a dual-host life cycle and its persistence.

* Corresponding author. Mailing address: Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave., Manhattan, KS 66506. Phone: (785) 532-4612. Fax: (785) 532-4851. E-mail: rganta{at}vet.k-state.edu.

{dagger} Kansas Agricultural Experiment Station contribution 05-9-J.

Editor: J. T. Barbieri

Infection and Immunity, January 2005, p. 79-87, Vol. 73, No. 1
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.1.79-87.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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