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Infection and Immunity, October 2005, p. 7018-7021, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7018-7021.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of New Antigens in Visceral Leishmaniasis by Expression Cloning and Immunoblotting with Sera of Kala-Azar Patients from Bihar, India{dagger}

Stephan M. Theinert,1 Rajatava Basu,2 Michael Forgber,1 Syamal Roy,2 Shyam Sundar,3 and Peter Walden1*

Clinical Research Group, Department of Dermatology, Charité University Medicine Berlin, Humboldt University, D-10089 Berlin, Germany,1 Indian Institute of Chemical Biology, Jadavpur, Calcutta 700032, India,2 Kala-Azar Medical Research Center, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India3

Received 20 August 2004/ Returned for modification 20 November 2004/ Accepted 4 July 2005

Sera of kala-azar patients from Bihar, India, were used to identify Leishmania donovani antigens encoded by a phage expression library. Ten antigens were identified, five of which have not been described as leishmania antigens before. The antigens specifically react with sera of leishmania-infected patients but not of toxoplasma- or plasmodium-infected patients.

* Corresponding author. Mailing address: Department of Dermatology, Charité University Medicine Berlin, Humboldt University, Schumannstr. 20/21, D-10117 Berlin, Germany. Phone: 49-30-450 518 031. Fax: 49-30-450 518 932. E-mail: peter.walden{at}charite.de.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: J. F. Urban, Jr.

Infection and Immunity, October 2005, p. 7018-7021, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7018-7021.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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