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Infection and Immunity, October 2005, p. 7022-7026, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7022-7026.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Digalactoside Expression in the Lipopolysaccharide of Haemophilus influenzae and Its Role in Intravascular Survival

Ruth Griffin,1* Chris D. Bayliss,1 Mark A. Herbert,1 Andrew D. Cox,2 Katherine Makepeace,1 James C. Richards,2 Derek W. Hood,1 and E. Richard Moxon1

Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom,1 Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, KIA 0R6Canada2

Received 15 March 2005/ Returned for modification 16 May 2005/ Accepted 16 June 2005

Digalactoside (gal{alpha}-1-4 galß) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

* Present address: Centre for Molecular Microbiology and Infection, Level 3, Flowers Building, Imperial College, London SW7 2AZ, United Kingdom. Phone: 0207 5943094. Fax: 0207 5943095. E-mail: r.griffin{at}imperial.ac.uk.

Editor: J. B. Bliska

Infection and Immunity, October 2005, p. 7022-7026, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7022-7026.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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