This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Griffin, R.
Right arrow Articles by Moxon, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Griffin, R.
Right arrow Articles by Moxon, E. R.

 Previous Article  |  Next Article 

Infection and Immunity, October 2005, p. 7022-7026, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7022-7026.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Digalactoside Expression in the Lipopolysaccharide of Haemophilus influenzae and Its Role in Intravascular Survival

Ruth Griffin,1* Chris D. Bayliss,1 Mark A. Herbert,1 Andrew D. Cox,2 Katherine Makepeace,1 James C. Richards,2 Derek W. Hood,1 and E. Richard Moxon1

Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom,1 Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, KIA 0R6Canada2

Received 15 March 2005/ Returned for modification 16 May 2005/ Accepted 16 June 2005

Digalactoside (gal{alpha}-1-4 galß) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

* Present address: Centre for Molecular Microbiology and Infection, Level 3, Flowers Building, Imperial College, London SW7 2AZ, United Kingdom. Phone: 0207 5943094. Fax: 0207 5943095. E-mail: r.griffin{at}imperial.ac.uk.

Editor: J. B. Bliska

Infection and Immunity, October 2005, p. 7022-7026, Vol. 73, No. 10
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.10.7022-7026.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Deadman, M. E., Hermant, P., Engskog, M., Makepeace, K., Moxon, E. R., Schweda, E. K. H., Hood, D. W. (2009). Lex2B, a Phase-Variable Glycosyltransferase, Adds either a Glucose or a Galactose to Haemophilus influenzae Lipopolysaccharide. Infect. Immun. 77: 2376-2384 [Abstract] [Full Text]  
  • Schweda, E. K.H., Twelkmeyer, B., Jianjun Li, (2008). Invited review: Profiling structural elements of short-chain lipopolysaccharide of non-typeable Haemophilus influenzae. Innate Immunity 14: 199-211 [Abstract]  
  • Rosadini, C. V., Wong, S. M. S., Akerley, B. J. (2008). The Periplasmic Disulfide Oxidoreductase DsbA Contributes to Haemophilus influenzae Pathogenesis. Infect. Immun. 76: 1498-1508 [Abstract] [Full Text]  
  • Dixon, K., Bayliss, C. D., Makepeace, K., Moxon, E. R., Hood, D. W. (2007). Identification of the Functional Initiation Codons of a Phase-Variable Gene of Haemophilus influenzae, lic2A, with the Potential for Differential Expression. J. Bacteriol. 189: 511-521 [Abstract] [Full Text]  
  • Erwin, A. L., Allen, S., Ho, D. K., Bonthius, P. J., Jarisch, J., Nelson, K. L., Tsao, D. L., Unrath, W. C. T., Watson, M. E. Jr., Gibson, B. W., Apicella, M. A., Smith, A. L. (2006). Role of lgtC in Resistance of Nontypeable Haemophilus influenzae Strain R2866 to Human Serum. Infect. Immun. 74: 6226-6235 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»