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Infection and Immunity, November 2005, p. 7259-7266, Vol. 73, No. 11
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.11.7259-7266.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

LruA and LruB, Novel Lipoproteins of Pathogenic Leptospira interrogans Associated with Equine Recurrent Uveitis

Ashutosh Verma,1 Sergey Artiushin,1 James Matsunaga,2,3 David A. Haake,2,3 and John F. Timoney1*

M. H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky 40546,1 Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California 90073,2 Department of Medicine, UCLA School of Medicine, Los Angeles, California 900953

Received 25 April 2005/ Returned for modification 18 May 2005/ Accepted 8 August 2005

Recurrent uveitis as a sequela to Leptospira infection is the most common infectious cause of blindness and impaired vision of horses worldwide. Leptospiral proteins expressed during prolonged survival in the eyes of horses with lesions of chronic uveitis were identified by screening a phage library of Leptospira interrogans DNA fragments with eye fluids from uveitic horses. Inserts of reactive phages encoded several known leptospiral proteins and two novel putative lipoproteins, LruA and LruB. LruA was intrinsically labeled during incubation of L. interrogans in medium containing [14C]palmitic acid, confirming that it is a lipoprotein. lruA and lruB were detected by Southern blotting in infectious Leptospira interrogans but not in nonpathogenic Leptospira biflexa. Fractionation data from cultured Leptospira indicate that LruA and LruB are localized in the inner membrane. Uveitic eye fluids contained significantly higher levels of immunoglobulin A (IgA) and IgG specific for each protein than did companion sera, indicating strong local antibody responses. Moreover, LruA- and LruB-specific antisera reacted with equine ocular components, suggesting an immunopathogenic role in leptospiral uveitis.

* Corresponding author. Mailing address: 325 M. H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546. Phone: (859) 257-4757, ext. 81106. Fax: (859) 257-5619. E-mail: jtimoney{at}uky.edu.

Editor: J. T. Barbieri

Infection and Immunity, November 2005, p. 7259-7266, Vol. 73, No. 11
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.11.7259-7266.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Verma, A., Rathinam, S. R., Priya, C. G., Muthukkaruppan, V. R., Stevenson, B., Timoney, J. F. (2008). LruA and LruB Antibodies in Sera of Humans with Leptospiral Uveitis. CVI 15: 1019-1023 [Abstract] [Full Text]  
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