This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental materials
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Radin, J. N.
Right arrow Articles by Tuomanen, E. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Radin, J. N.
Right arrow Articles by Tuomanen, E. I.

 Previous Article  |  Next Article 

Infection and Immunity, December 2005, p. 7827-7835, Vol. 73, No. 12
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.12.7827-7835.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

ß-Arrestin 1 Participates in Platelet-Activating Factor Receptor-Mediated Endocytosis of Streptococcus pneumoniae {dagger}

Jana N. Radin,1,2 Carlos J. Orihuela,1 Gopal Murti,3 Christopher Guglielmo,1 Peter J. Murray,1 and Elaine I. Tuomanen1,2*

Department of Infectious Diseases,1 Department of Molecular Biotechnology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105,3 Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 381632

Received 26 April 2005/ Returned for modification 10 June 2005/ Accepted 1 September 2005

Pneumococci traverse eukaryotic cells within vacuoles without intracytoplasmic multiplication. The platelet-activating factor receptor (PAFr) has been suggested as a portal of entry. Pneumococci colocalized with PAFr on endothelial cells and PAFr–/– mice showed a substantially impaired ability to support bacterial translocation, particularly from blood to brain. Pneumococci-induced colocalization of PAFr and ß-arrestin 1 at the plasma membrane of endothelial cells and PAFr-mediated pneumococcal uptake in transfected COS cells were greatly increased by cotransfection with the scaffold/adapter protein ß-arrestin 1. Activation of extracellular signal-regulated kinase kinases was required for uptake and was limited to the cytoplasmic compartment, consistent with activation by ß-arrestin rather than PAFr. Uptake of the pneumococcal vacuole involved clathrin, and half the bacteria proceeded into vacuoles marked by Rab5 and later Rab7, the classical route to the lysosome. Overexpression of ß-arrestin in endothelial cells decreased colocalization with Rab7. We conclude that the association of ß-arrestin with the PAFr contributes to successful translocation of pneumococci.

* Corresponding author. Mailing address: St. Jude Children's Research Hospital, Mailstop 320 IRC 8057, 332 N. Lauderdale Rd., Memphis, TN 38105. Phone: (901) 495-3486. Fax: (901) 495-3099. E-mail: elaine.tuomanen{at}stjude.org.

Editor: J. N. Weiser

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Infection and Immunity, December 2005, p. 7827-7835, Vol. 73, No. 12
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.12.7827-7835.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bergmann, S., Lang, A., Rohde, M., Agarwal, V., Rennemeier, C., Grashoff, C., Preissner, K. T., Hammerschmidt, S. (2009). Integrin-linked kinase is required for vitronectin-mediated internalization of Streptococcus pneumoniae by host cells. J. Cell Sci. 122: 256-267 [Abstract] [Full Text]  
  • Attali, C., Durmort, C., Vernet, T., Di Guilmi, A. M. (2008). The Interaction of Streptococcus pneumoniae with Plasmin Mediates Transmigration across Endothelial and Epithelial Monolayers by Intercellular Junction Cleavage. Infect. Immun. 76: 5350-5356 [Abstract] [Full Text]  
  • Leary, P. J., Rajasekaran, S., Morrison, R. R., Tuomanen, E. I., Chin, T. K., Hofmann, P. A. (2008). A cardioprotective role for platelet-activating factor through NOS-dependent S-nitrosylation. Am. J. Physiol. Heart Circ. Physiol. 294: H2775-H2784 [Abstract] [Full Text]  
  • Fillon, S., Soulis, K., Rajasekaran, S., Benedict-Hamilton, H., Radin, J. N., Orihuela, C. J., El Kasmi, K. C., Murti, G., Kaushal, D., Gaber, M. W., Weber, J. R., Murray, P. J., Tuomanen, E. I. (2006). Platelet-Activating Factor Receptor and Innate Immunity: Uptake of Gram-Positive Bacterial Cell Wall into Host Cells and Cell-Specific Pathophysiology. J. Immunol. 177: 6182-6191 [Abstract] [Full Text]  
  • Orihuela, C. J., Fillon, S., Smith-Sielicki, S. H., El Kasmi, K. C., Gao, G., Soulis, K., Patil, A., Murray, P. J., Tuomanen, E. I. (2006). Cell Wall-Mediated Neuronal Damage in Early Sepsis. Infect. Immun. 74: 3783-3789 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»