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Infection and Immunity, December 2005, p. 8002-8008, Vol. 73, No. 12
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.12.8002-8008.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

H2-M3 Major Histocompatibility Complex Class Ib-Restricted CD8 T Cells Induced by Salmonella enterica Serovar Typhimurium Infection Recognize Proteins Released by Salmonella Serovar Typhimurium

S. Ugrinovic,^1, C. G. Brooks,¹ J. Robson,¹ B. A. Blacklaws,² C. E. Hormaeche,^1,2, and J. H. Robinson^1*

Department of Microbiology and Immunology, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom,¹ Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom²

Received 26 April 2005/ Returned for modification 20 July 2005/ Accepted 23 August 2005

Salmonella enterica serovar Typhimurium causes a typhoid-like disease in mice which has been studied extensively as a model for typhoid fever in humans. CD8 T cells contribute to protection against S. enterica serovar Typhimurium in mice, but little is known about the specificity and major histocompatibility complex (MHC) restriction of the response. We report here that CD8 T-cell lines derived from S. enterica serovar Typhimurium-infected BALB/c mice lysed bone marrow macrophages infected with S. enterica serovar Typhimurium or pulsed with proteins from S. enterica serovar Typhimurium culture supernatants. Cytoxicity was beta-2-microglobulin dependent and largely TAP dependent, although not MHC class Ia restricted, as target cells of several different MHC haplotypes were lysed. The data suggested the participation of class Ib MHC molecules although no evidence for the presence of Qa1-restricted T cells could be found, unlike in previous reports. Instead, the T-cell lines lysed H2-M3-transfected fibroblasts infected with S. enterica serovar Typhimurium SL3261 or treated with Salmonella culture supernatants. Thus, this report increases the number of MHC class Ib antigen-presenting molecules known for Salmonella antigens to three: Qa-1, HLA-E, and now H2-M3. It also expands the range of pathogens that induce H2-M3-restricted CD8 T cells to include an example of gram-negative bacteria.

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* Corresponding author. Mailing address: Musculoskeletal Research Group, Clinical Medical Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom. Phone: 44 191 222 6976. Fax: 44 191 222 5455. E-mail: j.h.robinson{at}ncl.ac.uk.

Editor: F. C. Fang

Present address: Department of Clinical Biochemistry and Immunology, Addenbrookes Hospital, Hills Road, Cambridge CB2 2QQ, United Kingdom.

Deceased March 2005.

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Infection and Immunity, December 2005, p. 8002-8008, Vol. 73, No. 12
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.12.8002-8008.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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