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Infection and Immunity, December 2005, p. 8161-8166, Vol. 73, No. 12
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.12.8161-8166.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Antigenic Specificity of the Mucosal Antibody Response to Moraxella catarrhalis in Chronic Obstructive Pulmonary Disease

Timothy F. Murphy,1,3,4* Aimee L. Brauer,1 Christoph Aebi,5,6 and Sanjay Sethi2,4

Division of Infectious Diseases,1 Division of Pulmonary and Critical Care Medicine, Department of Medicine,2 Department of Microbiology, University at Buffalo, State University of New York,3 VA Western New York Healthcare System, Buffalo, New York,4 Institute for Infectious Diseases,5 Department of Pediatrics, University of Bern, Bern, Switzerland6

Received 15 July 2005/ Returned for modification 16 August 2005/ Accepted 7 September 2005

Moraxella catarrhalis is an important human mucosal pathogen causing otitis media in children and lower respiratory tract infection in adults with chronic obstructive pulmonary disease (COPD). Little is known about the mucosal antibody response to M. catarrhalis in adults with COPD. In this study, 10 pairs of well-characterized sputum supernatant samples from adults with COPD who had acquired and subsequently cleared M. catarrhalis from their respiratory tracts were studied in detail in an effort to begin to elucidate potentially protective immune responses. Flow cytometry analysis was used to study the distribution of immunoglobulin isotypes in paired preacquisition and postclearance sputum samples. The results showed that immunoglobulin A (IgA) is the predominant M. catarrhalis-specific immunoglobulin isotype and that the sputum IgA contains a secretory component, indicating that it is locally produced at the mucosal site. Most patients made new sputum IgA responses to the adhesins UspA1 and Hag, along with the surface protein UspA2. A smaller proportion of patients made new sputum IgA responses to the iron-regulated proteins TbpB and CopB and to lipooligosaccharide. These results have important implications in understanding the mucosal immune response to M. catarrhalis in the setting of COPD and in elucidating the elements of a protective immune response.

* Corresponding author. Mailing address: VA Western New York Healthcare System, Medical Research 151, 3495 Bailey Avenue, Buffalo, NY 14215. Phone: (716) 862-7874. Fax: (716) 862-6526. E-mail: murphyt{at}buffalo.edu.

Editor: D. L. Burns

Infection and Immunity, December 2005, p. 8161-8166, Vol. 73, No. 12
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.12.8161-8166.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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